biostudies-literatureUnknown20(5)Park MJTwo novel strains, MaLMAid0302T and SPO723T, isolated from coral and eelgrass, respectively, were distinguished from other Stappiaceae species based on phenotypic, biochemical, phylogenetic, and chemotaxonomic traits. Taxonomic challenges within the family Stappiaceae were addressed using a taxogenomic approach with iterative clustering, establishing an optimal average amino acid identity (AAI) threshold (71.92-72.88%) for genus delineation. This analysis led to major taxonomic revisions, including the establishment of new genera-Parapolycladidibacter, Astericibacter, Flexibacterium, Aliiroseibium, Laciiroseibium, Soliroseibium, Novilabrenzia, Litoriroseibium, and Algilabrenzia-as well as the reassignment of several species: Hongsoonwoonella albiluteola comb. nov., Parapolycladidibacter stPloS onee0322500https://www.ebi.ac.uk/biostudies/studies/S-EPMC12080928biostudies-literatureGenome-based reclassification of the family Stappiaceae and assessment of environmental forcing with the report of two novel taxa, Flexibacterium corallicola gen. nov., sp. nov., and Nesiotobacter zosterae sp. nov., isolated from coral and seagrass.PMC12080928Park MJJin HWoo SKim YJKwon KKKim JYu JZo YGfalseGenome-based reclassification of the family Stappiaceae and assessment of environmental forcing with the report of two novel taxa, Flexibacterium corallicola gen. nov., sp. nov., and Nesiotobacter zosterae sp. nov., isolated from coral and seagrass.Two novel strains, MaLMAid0302T and SPO723T, isolated from coral and eelgrass, respectively, were distinguished from other Stappiaceae species based on phenotypic, biochemical, phylogenetic, and chemotaxonomic traits. Taxonomic challenges within the family Stappiaceae were addressed using a taxogenomic approach with iterative clustering, establishing an optimal average amino acid identity (AAI) threshold (71.92-72.88%) for genus delineation. This analysis led to major taxonomic revisions, including the establishment of new genera-Parapolycladidibacter, Astericibacter, Flexibacterium, Aliiroseibium, Laciiroseibium, Soliroseibium, Novilabrenzia, Litoriroseibium, and Algilabrenzia-as well as the reassignment of several species: Hongsoonwoonella albiluteola comb. nov., Parapolycladidibacter st2025-01-01T00:00:00Z20252026-06-01T14:45:37.497Z2026-04-08T13:24:38.275ZS-EPMC120809284037311010.1371/journal.pone.0322500