biostudies-literatureWang LScientific Research Foundation for the Returned Overseas Chinese ScholarsTechnology Innovation Project of Colleges and Universities in Shanxi ProvinceMedical major Research program of ShanxiMajor Research and Development Program of Shanxi16849https://www.ebi.ac.uk/biostudies/studies/S-EPMC12081695biostudies-literatureUnknown15(1)Age-related macular degeneration (AMD) is the leading cause of vision loss in the elderly, and the role of chaperonin containing TCP1 subunit 2 (CCT2) remains unclear. This study aims to elucidate the mechanistic link between CCT2 and AMD, contributing to improved understanding and potential therapeutic strategies. Retinal and RPE-Choroid transcriptome array data from 130 AMD patients and 121 normal donors (GSE29801 dataset) were reanalyzed to assess CCT2 expression across different AMD subtypes, age groups, and genders. Single-sample gene set enrichment analysis was performed to explore correlations with autophagy-related genes and other established AMD causes. Additionally, CCT2 expression was validated in sodium iodate (NaIO₃)-induced 661 W cells (photoreceptor-like cells) using quantitScientific reportsClinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration.PMC12081695No2020RC22No. 2020-1952022L203No201903D321117Li MYZhou GHWang LXZhang RWang LfalseClinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration.Age-related macular degeneration (AMD) is the leading cause of vision loss in the elderly, and the role of chaperonin containing TCP1 subunit 2 (CCT2) remains unclear. This study aims to elucidate the mechanistic link between CCT2 and AMD, contributing to improved understanding and potential therapeutic strategies. Retinal and RPE-Choroid transcriptome array data from 130 AMD patients and 121 normal donors (GSE29801 dataset) were reanalyzed to assess CCT2 expression across different AMD subtypes, age groups, and genders. Single-sample gene set enrichment analysis was performed to explore correlations with autophagy-related genes and other established AMD causes. Additionally, CCT2 expression was validated in sodium iodate (NaIO₃)-induced 661 W cells (photoreceptor-like cells) using quantit2025-01-01T00:00:00Z2025 May2026-04-08T19:52:54.338Z2026-04-08T14:31:30.819ZS-EPMC120816954037473810.1038/s41598-025-01907-1