biostudies-literatureZhang HKnowledge Innovation Program of Wuhan -Basic Researche0321023https://www.ebi.ac.uk/biostudies/studies/S-EPMC12083875biostudies-literatureUnknown20(4)<h4>Importance</h4>Justicia procumbens L. contains lignans with remarkable antiplatelet aggregation effects. Its ethyl acetate extract has demonstrated the efficacy of inhibiting platelet aggregation in preliminary tests. Identifying key components, elucidating the antithrombotic mechanism, and evaluating its potential for treating thrombotic diseases are of great significance.<h4>Methods</h4>Chemical analysis was conducted on the ethyl acetate extract of J. procumbens. Animal models of carotid artery and venous thrombosis, combined with proteomics analysis, were employed to study the antithrombotic mechanism.<h4>Results</h4>Chemical analysis identified justicidin B (JB) and chinensinaphthol methyl ether (CME) as the major active antithrombotic constituents. The extract inhibited platelet PloS oneAntithrombotic effect and plasma pharmacochemistry of Justicia Procumbens L.PMC120838752022020801010419Liu YHu FZhang TZhang HXie ZLiu BChen SWu HfalseAntithrombotic effect and plasma pharmacochemistry of Justicia Procumbens L.<h4>Importance</h4>Justicia procumbens L. contains lignans with remarkable antiplatelet aggregation effects. Its ethyl acetate extract has demonstrated the efficacy of inhibiting platelet aggregation in preliminary tests. Identifying key components, elucidating the antithrombotic mechanism, and evaluating its potential for treating thrombotic diseases are of great significance.<h4>Methods</h4>Chemical analysis was conducted on the ethyl acetate extract of J. procumbens. Animal models of carotid artery and venous thrombosis, combined with proteomics analysis, were employed to study the antithrombotic mechanism.<h4>Results</h4>Chemical analysis identified justicidin B (JB) and chinensinaphthol methyl ether (CME) as the major active antithrombotic constituents. The extract inhibited platelet 2025-01-01T00:00:00Z20252026-06-02T20:54:22.276Z2026-05-28T03:06:30.623ZS-EPMC120838754030558510.1371/journal.pone.0321023