{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["28(5)"],"submitter":["Kang SW"],"funding":["Korea Ministry of Science and ICT"],"pubmed_abstract":["Peptidyl-prolyl <i>cis/trans</i> isomerases (PPIases) accelerate proline peptide bond isomerization, affecting substrate protein function. In this study, through RNAi-based behavioral screening of PPIases in <i>Drosophila melanogaster</i>, we identified <i>CG5808</i>, termed <i>Drosophila</i> peptidyl-prolyl <i>cis/trans</i> isomerase-like 4 (dPPIL4), as crucial for circadian rhythm regulation. Knockdown of <i>dppil4</i> in clock cells lengthened the circadian rhythm period and decreased rhythmicity, accompanied by a significant reduction of core clock protein PERIOD (PER). <i>d</i> <i>ppil4</i> knockdown downregulated <i>per</i> transcription and reduced phosphorylation at Ser5 in the RNA polymerase II C-terminal domain, critical for transcription elongation. In addition, dPPIL4 stabilize"],"journal":["iScience"],"pagination":["112457"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12084006"],"repository":["biostudies-literature"],"pubmed_title":["&lt;i&gt;Drosophila&lt;/i&gt; peptidyl-prolyl &lt;i&gt;cis/trans&lt;/i&gt; isomerase-like 4 regulates circadian rhythm by supporting high-amplitude oscillations of PERIOD."],"pmcid":["PMC12084006"],"pubmed_authors":["Kim EY","Kang SW","Lim SB","Ng JT","Tran HT","Lee G"],"additional_accession":[]},"is_claimable":false,"name":"&lt;i&gt;Drosophila&lt;/i&gt; peptidyl-prolyl &lt;i&gt;cis/trans&lt;/i&gt; isomerase-like 4 regulates circadian rhythm by supporting high-amplitude oscillations of PERIOD.","description":"Peptidyl-prolyl <i>cis/trans</i> isomerases (PPIases) accelerate proline peptide bond isomerization, affecting substrate protein function. In this study, through RNAi-based behavioral screening of PPIases in <i>Drosophila melanogaster</i>, we identified <i>CG5808</i>, termed <i>Drosophila</i> peptidyl-prolyl <i>cis/trans</i> isomerase-like 4 (dPPIL4), as crucial for circadian rhythm regulation. Knockdown of <i>dppil4</i> in clock cells lengthened the circadian rhythm period and decreased rhythmicity, accompanied by a significant reduction of core clock protein PERIOD (PER). <i>d</i> <i>ppil4</i> knockdown downregulated <i>per</i> transcription and reduced phosphorylation at Ser5 in the RNA polymerase II C-terminal domain, critical for transcription elongation. In addition, dPPIL4 stabilize","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-06-01T14:45:19.27Z","creation":"2026-04-08T13:24:00.27Z"},"accession":"S-EPMC12084006","cross_references":{"pubmed":["40384934"],"doi":["10.1016/j.isci.2025.112457"]}}