{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["170(6)"],"submitter":["Sutela S"],"funding":["Natural Resources Institute Finland"],"pubmed_abstract":["Using total RNA sequencing, we detected a novel RNA virus infecting a Finnish isolate of the fungal conifer pathogen Diplodia sapinea, and its genome sequence was verified by Sanger sequencing. This virus, which we have named \"Diplodia sapinea flexi-like virus 1\" (DsFLV1), has a 7,494-nucleotide-long positive-sense single-stranded RNA genome, excluding the poly(A) tail. The largest open reading frame (ORF1) encodes a replication-associated polyprotein (RP) that contains conserved domains characteristic of members of the order Tymovirales, including viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRP) domains. Four smaller ORFs were also identified. ORF2 potentially encodes a capsid protein, while the other three ORFs, initiated by alternative start c"],"journal":["Archives of virology"],"pagination":["131"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12084271"],"repository":["biostudies-literature"],"pubmed_title":["Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea."],"pmcid":["PMC12084271"],"pubmed_authors":["Trifkovic M","Sutela S","Terhonen E"],"additional_accession":[]},"is_claimable":false,"name":"Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea.","description":"Using total RNA sequencing, we detected a novel RNA virus infecting a Finnish isolate of the fungal conifer pathogen Diplodia sapinea, and its genome sequence was verified by Sanger sequencing. This virus, which we have named \"Diplodia sapinea flexi-like virus 1\" (DsFLV1), has a 7,494-nucleotide-long positive-sense single-stranded RNA genome, excluding the poly(A) tail. The largest open reading frame (ORF1) encodes a replication-associated polyprotein (RP) that contains conserved domains characteristic of members of the order Tymovirales, including viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRP) domains. Four smaller ORFs were also identified. ORF2 potentially encodes a capsid protein, while the other three ORFs, initiated by alternative start c","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-04-08T19:53:47.964Z","creation":"2026-04-08T14:32:15.765Z"},"accession":"S-EPMC12084271","cross_references":{"pubmed":["40379837"],"doi":["10.1007/s00705-025-06313-6"]}}