{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hong SR"],"funding":["National Science and Technology Council","National Science and Technology Council (NSTC)"],"pagination":["2976-2996"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12084555"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["44(10)"],"pubmed_abstract":["Centrosomes are tubulin-based organelles that undergo glutamylation, a post-translational modification that conjugates glutamic acid residues to tubulins. Although centrosomal glutamylation has been known for several decades, how this modification regulates centrosome structure and function remains unclear. To address this long-standing issue, we developed a method to spatiotemporally reduce centrosomal glutamylation by recruiting an engineered deglutamylase to centrosomes. We found that centrosome structure remains largely unaffected by centrosomal hypoglutamylation. Intriguingly, glutamylation physically recruits, via electrostatic forces, the NEDD1/CEP192/γ-tubulin complex to centrosomes, ensuring microtubule nucleation and proper trafficking of centriolar satellites. The consequent def"],"journal":["The EMBO journal"],"pubmed_title":["Glutamylation of centrosomes ensures their function by recruiting microtubule nucleation factors."],"pmcid":["PMC12084555"],"funding_grant_id":["113-2628-B-007-003","08-2311-B-007-002-MY3","114-2823-8-007 -003","113-2320-B-A49-018-MY3","112-2628-B-A49-009-MY3"],"pubmed_authors":["Chou PC","Lin IH","Liao KJ","Yeh HW","Sharma L","Chang YC","Cheng HC","Wang WJ","Yang WT","Lin YC","Lu JC","Li RY","Hong SR","Song CS","Wu BH","Wang LH","Chen SC","Chuang YC"],"additional_accession":[]},"is_claimable":false,"name":"Glutamylation of centrosomes ensures their function by recruiting microtubule nucleation factors.","description":"Centrosomes are tubulin-based organelles that undergo glutamylation, a post-translational modification that conjugates glutamic acid residues to tubulins. Although centrosomal glutamylation has been known for several decades, how this modification regulates centrosome structure and function remains unclear. To address this long-standing issue, we developed a method to spatiotemporally reduce centrosomal glutamylation by recruiting an engineered deglutamylase to centrosomes. We found that centrosome structure remains largely unaffected by centrosomal hypoglutamylation. Intriguingly, glutamylation physically recruits, via electrostatic forces, the NEDD1/CEP192/γ-tubulin complex to centrosomes, ensuring microtubule nucleation and proper trafficking of centriolar satellites. The consequent def","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-06-01T14:45:44.763Z","creation":"2026-04-08T13:25:00.38Z"},"accession":"S-EPMC12084555","cross_references":{"pubmed":["40229407"],"doi":["10.1038/s44318-025-00435-y"]}}