biostudies-literatureAn RNational Natural Science Foundation of China181https://www.ebi.ac.uk/biostudies/studies/S-EPMC12090600biostudies-literatureUnknown18(1)<h4>Background</h4>Toxoplasma gondii, an obligate intracellular parasite, has developed sophisticated ways to manipulate host immunity, resulting in long-lasting infection and causing serious public health problems in humans and animals. T. gondii type II is the type most frequently associated with human diseases, but the mechanism remains unclear. Toxoplasma calcium-dependent protein kinase 3(CDPK3), a protein located on the T. gondii parasite periphery, is highly expressed in type II strains. Although TgCDPK3 regulates parasite egress from host cells, calcium-based infiltration, and development of tissue cysts, the host target proteins that it modulates are still unclear.<h4>Methods</h4>Firstly, mass spectrometry was used to analyze proteins that selectively bind to TgCDPK3. SubsequentlyParasites & vectorsToxoplasma calcium-dependent protein kinases 3 mediates M1 macrophage polarization by targeting host Arginase-1.PMC1209060082072300An RLiu XDai NLi FChen LDu JLiu FCai HfalseToxoplasma calcium-dependent protein kinases 3 mediates M1 macrophage polarization by targeting host Arginase-1.<h4>Background</h4>Toxoplasma gondii, an obligate intracellular parasite, has developed sophisticated ways to manipulate host immunity, resulting in long-lasting infection and causing serious public health problems in humans and animals. T. gondii type II is the type most frequently associated with human diseases, but the mechanism remains unclear. Toxoplasma calcium-dependent protein kinase 3(CDPK3), a protein located on the T. gondii parasite periphery, is highly expressed in type II strains. Although TgCDPK3 regulates parasite egress from host cells, calcium-based infiltration, and development of tissue cysts, the host target proteins that it modulates are still unclear.<h4>Methods</h4>Firstly, mass spectrometry was used to analyze proteins that selectively bind to TgCDPK3. Subsequently2025-01-01T00:00:00Z2025 May2026-04-08T19:24:29.546Z2026-04-08T13:30:38.234ZS-EPMC120906004039472110.1186/s13071-025-06799-8