{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Di Cianni N"],"funding":["DFG"],"pagination":["uqaf008"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12091111"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6"],"pubmed_abstract":["<i>Haloferax volcanii</i> harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3, and Halfvol4. In this study, we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (∆Halfvol1-4) and a wild-type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ∆Halfvol1-4. Furthermore, ∆Halfvol1-4 cells show an elongated cell shape and a higher resistance to H<sub>2</sub>O<sub>2</sub> stress compared to the wild type. RNA-seq also revealed downregulation of CRISPR arrays in the provirus-free strain. Circularised genomes of Halfvol1, Halfvol2, and Halfvol3 were found in the culture supernatant of the wild-type strain. This confirms excision of the pro"],"journal":["microLife"],"pubmed_title":["Provirus deletion from &lt;i&gt;Haloferax volcanii&lt;/i&gt; affects motility, stress resistance, and CRISPR RNA expression."],"pmcid":["PMC12091111"],"funding_grant_id":["SPP2141","Ma1538/25-2","Ma1538/27-1"],"pubmed_authors":["Walther P","Allers T","Urlaub H","Marchfelder A","Di Cianni N","Konig S","Mitchell L","Raabe M","Grafenhan T","Erdmann S","Dyall-Smith M","Read C","Bischler T","Brendel J","Pfeiffer F","Bolsinger S"],"additional_accession":[]},"is_claimable":false,"name":"Provirus deletion from &lt;i&gt;Haloferax volcanii&lt;/i&gt; affects motility, stress resistance, and CRISPR RNA expression.","description":"<i>Haloferax volcanii</i> harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3, and Halfvol4. In this study, we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (∆Halfvol1-4) and a wild-type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ∆Halfvol1-4. Furthermore, ∆Halfvol1-4 cells show an elongated cell shape and a higher resistance to H<sub>2</sub>O<sub>2</sub> stress compared to the wild type. RNA-seq also revealed downregulation of CRISPR arrays in the provirus-free strain. Circularised genomes of Halfvol1, Halfvol2, and Halfvol3 were found in the culture supernatant of the wild-type strain. This confirms excision of the pro","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-04-08T18:39:13.835Z","creation":"2026-04-08T09:51:35.475Z"},"accession":"S-EPMC12091111","cross_references":{"pubmed":["40395997"],"doi":["10.1093/femsml/uqaf008"]}}