{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Liu B"],"pubmed_abstract":["Cisplatin (DDP) remains a cornerstone therapy for osteosarcoma (OS); however, pervasive resistance severely limits its clinical efficacy and worsens patient outcomes. Developing strategies to enhance the chemotherapeutic responsiveness of OS cells is therefore of critical importance. Here, we conducted a kinome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screen, coupled with transcriptome sequencing, to identify regulators of DDP sensitivity. This approach revealed protein kinase membrane-associated tyrosine/threonine 1 (PKMYT1) as a key regulator of DDP sensitivity in OS. Subsequent analysis of patient-derived clinical specimens, along with in vitro functional assays, demonstrated that DDP treatment induces the activation of PKMYT1 in OS cells. Importantly, PKM"],"journal":["Signal transduction and targeted therapy"],"pagination":["165"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12092789"],"repository":["biostudies-literature"],"pubmed_title":["PKMYT1 kinase ameliorates cisplatin sensitivity in osteosarcoma."],"pmcid":["PMC12092789"],"pubmed_authors":["Jin X","Tu C","Xu R","Wan L","Fu Z","Xu H","Feng C","Li W","Zhang W","He S","Liu Z","Li Z","Liu B"],"additional_accession":[]},"is_claimable":false,"name":"PKMYT1 kinase ameliorates cisplatin sensitivity in osteosarcoma.","description":"Cisplatin (DDP) remains a cornerstone therapy for osteosarcoma (OS); however, pervasive resistance severely limits its clinical efficacy and worsens patient outcomes. Developing strategies to enhance the chemotherapeutic responsiveness of OS cells is therefore of critical importance. Here, we conducted a kinome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screen, coupled with transcriptome sequencing, to identify regulators of DDP sensitivity. This approach revealed protein kinase membrane-associated tyrosine/threonine 1 (PKMYT1) as a key regulator of DDP sensitivity in OS. Subsequent analysis of patient-derived clinical specimens, along with in vitro functional assays, demonstrated that DDP treatment induces the activation of PKMYT1 in OS cells. Importantly, PKM","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-06-01T10:22:28.812Z","creation":"2026-04-08T11:22:55.009Z"},"accession":"S-EPMC12092789","cross_references":{"pubmed":["40393983"],"doi":["10.1038/s41392-025-02250-7"]}}