biostudies-literatureJames Mthe Agence Nationale de la Recherche (ANR)e70287https://www.ebi.ac.uk/biostudies/studies/S-EPMC12092965biostudies-literatureUnknown177(3)In winter oilseed rape (Brassica napus L.), vernalization, prolonged cold exposure, is essential for spring flowering. Although transcriptomic changes in leaves during vernalization are studied, the taproot, a key storage organ, remains unexplored. Recently, high nitrogen (N) and carbon (C) compound levels were observed in the taproot post-vernalization, suggesting potential metabolic activities in this organ during this period. To decipher this, an integrative study combining morphological, ionomic, proteomic, and targeted biochemical analysis was conducted. This study revealed that the taproot is the only compartment that shows net gain in biomass during vernalization and confirmed its role in storing C and N reserves. A comparative proteomic analysis between the beginning and the end ofPhysiologia plantarumThe Taproot Acts as a Storage Organ During Rapeseed Vernalization.PMC12092965ANR-19-CE14-0009-02Trouverie JJames MGoux DJing LMasclaux-Daubresse CEtienne PfalseThe Taproot Acts as a Storage Organ During Rapeseed Vernalization.In winter oilseed rape (Brassica napus L.), vernalization, prolonged cold exposure, is essential for spring flowering. Although transcriptomic changes in leaves during vernalization are studied, the taproot, a key storage organ, remains unexplored. Recently, high nitrogen (N) and carbon (C) compound levels were observed in the taproot post-vernalization, suggesting potential metabolic activities in this organ during this period. To decipher this, an integrative study combining morphological, ionomic, proteomic, and targeted biochemical analysis was conducted. This study revealed that the taproot is the only compartment that shows net gain in biomass during vernalization and confirmed its role in storing C and N reserves. A comparative proteomic analysis between the beginning and the end of2025-01-01T00:00:00Z2025 May-Jun2026-06-01T10:23:49.841Z2026-04-08T11:26:05.87ZS-EPMC120929654039481910.1111/ppl.70287