{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cui JH"],"funding":["Canada Research Chair Tier II","University of British Columbia","Canadian Institutes for Health Research","The Stem Cell Network Early Career Researcher Jump Start Awards Program","National Sciences and Engineering Research Counci"],"pagination":["gkaf436"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12093143"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(9)"],"pubmed_abstract":["The TATA box-binding protein (TBP) is an evolutionarily conserved basal transcription factor common in the pre-initiation complex of all three eukaryotic RNA polymerases (RNA Pols). Despite their high conservation, homologous TBPs exhibit species- and tissue-specific functions that may contribute to the increasingly complex gene expression regulation across evolutionary time. To determine the molecular mechanisms of species- and tissue-specificity for homologous TBPs, we examined the ability of yeast TBP and murine TBP paralogs to replace the endogenous TBP in mouse embryonic stem cells (mESCs). We show that, despite the high conservation in the DNA-binding domain among the homologs, they cannot fully rescue the lethality of TBP depletion in mESCs, which correlates with their inability to "],"journal":["Nucleic acids research"],"pubmed_title":["Functional divergence of TBP homologs through distinct DNA-binding dynamics."],"pmcid":["PMC12093143"],"funding_grant_id":["ECR-C4R1-11","PJT-191938","GPIN-2020-06106","PJT-162289","CRC-RS 2021-00294"],"pubmed_authors":["Cui JH","Kwan JZJ","Nguyen TF","Faghihi A","Teves SS"],"additional_accession":[]},"is_claimable":false,"name":"Functional divergence of TBP homologs through distinct DNA-binding dynamics.","description":"The TATA box-binding protein (TBP) is an evolutionarily conserved basal transcription factor common in the pre-initiation complex of all three eukaryotic RNA polymerases (RNA Pols). Despite their high conservation, homologous TBPs exhibit species- and tissue-specific functions that may contribute to the increasingly complex gene expression regulation across evolutionary time. To determine the molecular mechanisms of species- and tissue-specificity for homologous TBPs, we examined the ability of yeast TBP and murine TBP paralogs to replace the endogenous TBP in mouse embryonic stem cells (mESCs). We show that, despite the high conservation in the DNA-binding domain among the homologs, they cannot fully rescue the lethality of TBP depletion in mESCs, which correlates with their inability to ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-04-08T18:34:59.143Z","creation":"2026-04-08T09:30:36.319Z"},"accession":"S-EPMC12093143","cross_references":{"pubmed":["40396489"],"doi":["10.1093/nar/gkaf436"]}}