{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sheng Y"],"funding":["National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","National Natural Science Foundation of China","Foundation for Innovative Research Groups of the National Natural Science Foundation of China","Jiangsu Basic Research Center for Synthetic Biology","National Science Fund for Excellent Young Scholars","Natural Science Foundation of Jiangsu Province"],"pagination":["e70145"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12093341"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(5)"],"pubmed_abstract":["Creatine (CR) is a naturally occurring amino acid derivative that plays a key role in cellular energy homeostasis, which has wide-ranging applications in food and medicine. Currently, the lack of green and sustainable CR biomanufacturing methods has led to reliance on chemical methods for industrial CR synthesis. This study presents a biological approach to synthesising CR using whole-cell catalysis by engineered Escherichia coli. First, through screening of critical enzymes from different sources and dual-enzyme co-expression strategies, arginine: glycine amidinotransferase (AGAT) from Amycolatopsis kentuckyensis and guanidinoacetate N-methyltransferase (GAMT) from Mus caroli were introduced to construct the CR biosynthesis pathway, yielding 0.83 g/L CR production. Then, the expression le"],"journal":["Microbial biotechnology"],"pubmed_title":["Reconstitution of Methionine Cycle With ATP Regeneration for Whole-Cell Catalysis of Creatine Production in Engineered Escherichia coli."],"pmcid":["PMC12093341"],"funding_grant_id":["BK20233003","32172349","32021005","BK20202002","32222069"],"pubmed_authors":["Liu Y","Sheng Y","Li J","Liu L","Chen J","Lv X","Du G","Zhang L","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Reconstitution of Methionine Cycle With ATP Regeneration for Whole-Cell Catalysis of Creatine Production in Engineered Escherichia coli.","description":"Creatine (CR) is a naturally occurring amino acid derivative that plays a key role in cellular energy homeostasis, which has wide-ranging applications in food and medicine. Currently, the lack of green and sustainable CR biomanufacturing methods has led to reliance on chemical methods for industrial CR synthesis. This study presents a biological approach to synthesising CR using whole-cell catalysis by engineered Escherichia coli. First, through screening of critical enzymes from different sources and dual-enzyme co-expression strategies, arginine: glycine amidinotransferase (AGAT) from Amycolatopsis kentuckyensis and guanidinoacetate N-methyltransferase (GAMT) from Mus caroli were introduced to construct the CR biosynthesis pathway, yielding 0.83 g/L CR production. Then, the expression le","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-07-15T05:03:42.722Z","creation":"2026-06-30T03:08:25.259Z"},"accession":"S-EPMC12093341","cross_references":{"pubmed":["40396566"],"doi":["10.1111/1751-7915.70145"]}}