{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He X"],"funding":["National High Level Hospital Clinical Research Funding (Scientific and Technological Achievements Transformation Incubation Guidance Fund Project of Peking University First Hospital)","Beijing Nova Program","the National Natural Science Foundation of China","Peking University Clinical Scientist Training Program"],"pagination":["17673"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12095773"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Patients with ulcerative colitis (UC) have a significantly impaired intestinal barrier. Hydrogen Sulfide (H<sub>2</sub>S) is a gaseous mediator that makes notable contributions in a variety of diseases, such as reducing inflammatory response in colitis. The experimental content includes the establishment of a mouse DSS-induced colitis mouse model, mouse colon epithelial organoids culture, H&E staining and mass spectrometry analysis. We recognized that exogenous H<sub>2</sub>S donor-GYY4137 significantly alleviated the symptoms in UC mice models and maintained Minichromosome Maintenance Complex Component 2 (MCM2) expression. CBS knockdown reduced the expression of sulfhydrated Ribosomal protein S20 (RPS20-ssh) and MCM2 in the mouse colon. Cell experiments indicated that the expression of RP"],"journal":["Scientific reports"],"pubmed_title":["Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis."],"pmcid":["PMC12095773"],"funding_grant_id":["BMU2023PYJH001","32370837","20230484244","2023CX04"],"pubmed_authors":["Liu X","Liu Y","Li J","Wang P","Xue B","Zhu J","Huang Z","You M","He X","Wang X","Chen S"],"additional_accession":[]},"is_claimable":false,"name":"Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis.","description":"Patients with ulcerative colitis (UC) have a significantly impaired intestinal barrier. Hydrogen Sulfide (H<sub>2</sub>S) is a gaseous mediator that makes notable contributions in a variety of diseases, such as reducing inflammatory response in colitis. The experimental content includes the establishment of a mouse DSS-induced colitis mouse model, mouse colon epithelial organoids culture, H&E staining and mass spectrometry analysis. We recognized that exogenous H<sub>2</sub>S donor-GYY4137 significantly alleviated the symptoms in UC mice models and maintained Minichromosome Maintenance Complex Component 2 (MCM2) expression. CBS knockdown reduced the expression of sulfhydrated Ribosomal protein S20 (RPS20-ssh) and MCM2 in the mouse colon. Cell experiments indicated that the expression of RP","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-06-03T02:42:50.5Z","creation":"2026-04-23T03:11:37.154Z"},"accession":"S-EPMC12095773","cross_references":{"pubmed":["40399407"],"doi":["10.1038/s41598-025-02268-5"]}}