biostudies-literatureHe XNational High Level Hospital Clinical Research Funding (Scientific and Technological Achievements Transformation Incubation Guidance Fund Project of Peking University First Hospital)Beijing Nova Programthe National Natural Science Foundation of ChinaPeking University Clinical Scientist Training Program17673https://www.ebi.ac.uk/biostudies/studies/S-EPMC12095773biostudies-literatureUnknown15(1)Patients with ulcerative colitis (UC) have a significantly impaired intestinal barrier. Hydrogen Sulfide (H<sub>2</sub>S) is a gaseous mediator that makes notable contributions in a variety of diseases, such as reducing inflammatory response in colitis. The experimental content includes the establishment of a mouse DSS-induced colitis mouse model, mouse colon epithelial organoids culture, H&E staining and mass spectrometry analysis. We recognized that exogenous H<sub>2</sub>S donor-GYY4137 significantly alleviated the symptoms in UC mice models and maintained Minichromosome Maintenance Complex Component 2 (MCM2) expression. CBS knockdown reduced the expression of sulfhydrated Ribosomal protein S20 (RPS20-ssh) and MCM2 in the mouse colon. Cell experiments indicated that the expression of RPScientific reportsHydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis.PMC12095773BMU2023PYJH00132370837202304842442023CX04Liu XLiu YLi JWang PXue BZhu JHuang ZYou MHe XWang XChen SfalseHydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis.Patients with ulcerative colitis (UC) have a significantly impaired intestinal barrier. Hydrogen Sulfide (H<sub>2</sub>S) is a gaseous mediator that makes notable contributions in a variety of diseases, such as reducing inflammatory response in colitis. The experimental content includes the establishment of a mouse DSS-induced colitis mouse model, mouse colon epithelial organoids culture, H&E staining and mass spectrometry analysis. We recognized that exogenous H<sub>2</sub>S donor-GYY4137 significantly alleviated the symptoms in UC mice models and maintained Minichromosome Maintenance Complex Component 2 (MCM2) expression. CBS knockdown reduced the expression of sulfhydrated Ribosomal protein S20 (RPS20-ssh) and MCM2 in the mouse colon. Cell experiments indicated that the expression of RP2025-01-01T00:00:00Z2025 May2026-06-03T02:42:50.5Z2026-04-23T03:11:37.154ZS-EPMC120957734039940710.1038/s41598-025-02268-5