biostudies-literatureSahota ACancer Research UKEuropean Research CouncilMedical Research CouncilWellcome TrustAcademy of Medical SciencesEngineering and Physical Sciences Research Council18522-18533https://www.ebi.ac.uk/biostudies/studies/S-EPMC12096465biostudies-literatureUnknown19(19)Emerging techniques for mapping mRNAs within the subcellular compartments of live cells hold great promise for advancing our understanding of the spatial distribution of transcripts and enabling the study of single-cell dynamics in health and disease. This is particularly critical for polarized cells, such as neurons, where mRNA compartmentalization is essential for regulating gene expression, and defects in these localization mechanisms are linked to numerous neurological disorders. However, many subcellular analysis techniques require a compromise between subcellular precision, live-cell measurements, and nondestructive access to single cells in their native microenvironment. To overcome these challenges, we employ a single-cell technology that we have recently developed, the nanotweezerACS nanoSpatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers.PMC12096465EP/T022000/1SBF008\1179724300EP/V049070/1CC2206875525Kittler JTEdel JBKwan ZClaxton SPaulose Nadappuram BLesept FSahota AHowden JHIvanov APDevine MJfalseSpatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers.Emerging techniques for mapping mRNAs within the subcellular compartments of live cells hold great promise for advancing our understanding of the spatial distribution of transcripts and enabling the study of single-cell dynamics in health and disease. This is particularly critical for polarized cells, such as neurons, where mRNA compartmentalization is essential for regulating gene expression, and defects in these localization mechanisms are linked to numerous neurological disorders. However, many subcellular analysis techniques require a compromise between subcellular precision, live-cell measurements, and nondestructive access to single cells in their native microenvironment. To overcome these challenges, we employ a single-cell technology that we have recently developed, the nanotweezer2025-01-01T00:00:00Z2025 May2026-07-15T11:12:37.621Z2026-07-05T03:08:36.612ZS-EPMC120964654032674010.1021/acsnano.5c02056