{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu L"],"funding":["European Research Council","National Science Foundation of China","Royal Society, UK"],"pagination":["137"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12096798"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(1)"],"pubmed_abstract":["<h4>Background</h4>The H2A.Z histone variant is highly enriched over gene bodies, playing an essential role in several genome-templated processes, including transcriptional regulation and epigenetic patterning across eukaryotes. Deposition of H2A.Z is mediated by the SWR1 remodeling complex. How SWR1 is directed to gene bodies is largely unknown.<h4>Results</h4>Here, we show that ALFIN-LIKE (AL) proteins are responsible for H2A.Z gene body patterning in Arabidopsis. AL proteins encode H3K4me3-binding PHD domains, and by ChIP-seq, we confirm preferential binding of AL5 to H3K4me3 over H3K4me1/2 in planta. We observe a global reduction in H2A.Z in al septuple mutants (al7m), especially over H3K4me3-enriched genic regions. While MBD9 recruits SWR1 to nucleosome-free regions, ALs act non-redun"],"journal":["Genome biology"],"pubmed_title":["H3K4me3 binding ALFIN-LIKE proteins recruit SWR1 for gene-body deposition of H2A.Z."],"pmcid":["PMC12096798"],"funding_grant_id":["321703059 and 32370580","URF\\R1\\201016","EP/X025306/1"],"pubmed_authors":["Xu L","Adamkova V","Li X","Harris CJ","Wang Y","Hu Q","Ausin I","Xu J"],"additional_accession":[]},"is_claimable":false,"name":"H3K4me3 binding ALFIN-LIKE proteins recruit SWR1 for gene-body deposition of H2A.Z.","description":"<h4>Background</h4>The H2A.Z histone variant is highly enriched over gene bodies, playing an essential role in several genome-templated processes, including transcriptional regulation and epigenetic patterning across eukaryotes. Deposition of H2A.Z is mediated by the SWR1 remodeling complex. How SWR1 is directed to gene bodies is largely unknown.<h4>Results</h4>Here, we show that ALFIN-LIKE (AL) proteins are responsible for H2A.Z gene body patterning in Arabidopsis. AL proteins encode H3K4me3-binding PHD domains, and by ChIP-seq, we confirm preferential binding of AL5 to H3K4me3 over H3K4me1/2 in planta. We observe a global reduction in H2A.Z in al septuple mutants (al7m), especially over H3K4me3-enriched genic regions. While MBD9 recruits SWR1 to nucleosome-free regions, ALs act non-redun","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-07-15T08:28:02.195Z","creation":"2026-07-01T03:12:05.838Z"},"accession":"S-EPMC12096798","cross_references":{"pubmed":["40399998"],"doi":["10.1186/s13059-025-03605-7"]}}