biostudies-literatureLlontop-Bernabe KSCSIC, SPAINPROCIENCIA-CONCYTEC, Peru32https://www.ebi.ac.uk/biostudies/studies/S-EPMC12101353biostudies-literatureUnknown14(2)The growing demand for functional foods has driven the search for bioactive compounds derived from plant proteins. <i>Lupinus mutabilis</i> "Tarwi", a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bioactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functionally classified into three groups: cellular component (35.57%), molecular function (33.45%), and biological process (30.97%). The isolated AF was hydrolysed with the native protease PC7 (HAP), optimizing the E/S ratio and time parameters. Additionally, sequential hydrolysis of the PC7 protease andBiotech (Basel (Switzerland))Production of Multifunctional Hydrolysates from the &lt;i&gt;Lupinus mutabilis&lt;/i&gt; Protein Using a &lt;i&gt;Micrococcus&lt;/i&gt; sp. PC7 Protease.PMC12101353Contract No. PE501078969-2022-PROCIENCIAProject COOPB23020Zavaleta AIIntiquilla APaterson SJimenez-Aliaga KHernandez-Ledesma BLlontop-Bernabe KSSantos MRamirez-Veliz CfalseProduction of Multifunctional Hydrolysates from the &lt;i&gt;Lupinus mutabilis&lt;/i&gt; Protein Using a &lt;i&gt;Micrococcus&lt;/i&gt; sp. PC7 Protease.The growing demand for functional foods has driven the search for bioactive compounds derived from plant proteins. <i>Lupinus mutabilis</i> "Tarwi", a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bioactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functionally classified into three groups: cellular component (35.57%), molecular function (33.45%), and biological process (30.97%). The isolated AF was hydrolysed with the native protease PC7 (HAP), optimizing the E/S ratio and time parameters. Additionally, sequential hydrolysis of the PC7 protease and2025-01-01T00:00:00Z2025 Apr2026-05-26T14:00:47.571Z2026-05-24T03:07:04.404ZS-EPMC121013534040748710.3390/biotech14020032