biostudies-literatureLian Qthe Fujian Provincial Clinical Medical Research Center for First Aid and Rehabilitation in Orthopedic Traumathe Youth Cultivation Program for Medical-Engineering Interdisciplinary Research at the Third Affiliated Hospital of Naval Medical Universitythe National Natural Science Foundation of China159https://www.ebi.ac.uk/biostudies/studies/S-EPMC12102912biostudies-literatureUnknown44(1)<h4>Background</h4>The prognosis of osteosarcoma has improved little over the past few decades, with radioresistance being a contributing factor. Effective radiosensitizing targets and novel mechanisms for treating osteosarcoma are urgently needed. Research on the impact of regulating differentiation levels on the radiosensitivity of malignant tumors is limited. This study aimed to explore the efficacy of ITGB3 as a novel radiosensitizing target in osteosarcoma and to explore whether the modulation of osteogenic differentiation plays a role in mediating the radiosensitizing effect.<h4>Methods</h4>RNA sequencing was utilized to screen for potential targets that affect the radiosensitivity of osteosarcoma. In vitro assays examining cell viability, apoptosis, proliferation, migration, and invJournal of experimental & clinical cancer research : CREnhancing radiosensitivity of osteosarcoma by ITGB3 knockdown: a mechanism linked to enhanced osteogenic differentiation status through JNK/c-JUN/RUNX2 pathway activation.PMC121029122022YGJC003821713832020Y2014Liu HLi JQiao SWang BDai PJiang XLiu CLuo CZhou HWang ZLian QZhao HfalseEnhancing radiosensitivity of osteosarcoma by ITGB3 knockdown: a mechanism linked to enhanced osteogenic differentiation status through JNK/c-JUN/RUNX2 pathway activation.<h4>Background</h4>The prognosis of osteosarcoma has improved little over the past few decades, with radioresistance being a contributing factor. Effective radiosensitizing targets and novel mechanisms for treating osteosarcoma are urgently needed. Research on the impact of regulating differentiation levels on the radiosensitivity of malignant tumors is limited. This study aimed to explore the efficacy of ITGB3 as a novel radiosensitizing target in osteosarcoma and to explore whether the modulation of osteogenic differentiation plays a role in mediating the radiosensitizing effect.<h4>Methods</h4>RNA sequencing was utilized to screen for potential targets that affect the radiosensitivity of osteosarcoma. In vitro assays examining cell viability, apoptosis, proliferation, migration, and inv2025-01-01T00:00:00Z2025 May2026-07-15T12:36:51.252Z2026-07-04T03:14:14.097ZS-EPMC121029124041089710.1186/s13046-025-03417-4