{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rodriguez-Rodriguez A"],"funding":["Consejo Nacional de Humanidades, Ciencias y Tecnologías"],"pagination":["725"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12110239"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(10)"],"pubmed_abstract":["The amplitude of the phasic output of thyrotropin-releasing hormone (TRH) into the hypothalamus-pituitary portal capillaries is likely controlled by the TRH-degrading ectoenzyme (TRH-DE) expressed on the surface of median eminence (ME) β2-tanycytes. To extend this hypothesis, we performed experiments on adult rodents reared in standard conditions. TRH-DE was close to the putative sites of TRH release in the male rat external layer of the ME. In global <i>Trhde</i> knockout mice, basal hypothalamus-pituitary-thyroid (HPT) axis parameters were not altered but we detected an increased vimentin (a tanycyte marker) positive coverage of the portal vessels. We then overexpressed TRH-DE or a dominant negative isoform by microinjection of adeno-associated virus 1 (AAV1) vectors into the third ventr"],"journal":["Cells"],"pubmed_title":["Hypothalamic Median Eminence Thyrotropin-Releasing Hormone-Degrading Ectoenzyme Activity Is Dispensable for Basal Thyroid Axis Activity in Lean Rodents."],"pmcid":["PMC12110239"],"funding_grant_id":["2015-01-562","CBF2023-2024-732","2014-254960"],"pubmed_authors":["Cote-Velez A","Joseph-Bravo P","Charli JL","Uribe RM","Rodriguez-Rodriguez A"],"additional_accession":[]},"is_claimable":false,"name":"Hypothalamic Median Eminence Thyrotropin-Releasing Hormone-Degrading Ectoenzyme Activity Is Dispensable for Basal Thyroid Axis Activity in Lean Rodents.","description":"The amplitude of the phasic output of thyrotropin-releasing hormone (TRH) into the hypothalamus-pituitary portal capillaries is likely controlled by the TRH-degrading ectoenzyme (TRH-DE) expressed on the surface of median eminence (ME) β2-tanycytes. To extend this hypothesis, we performed experiments on adult rodents reared in standard conditions. TRH-DE was close to the putative sites of TRH release in the male rat external layer of the ME. In global <i>Trhde</i> knockout mice, basal hypothalamus-pituitary-thyroid (HPT) axis parameters were not altered but we detected an increased vimentin (a tanycyte marker) positive coverage of the portal vessels. We then overexpressed TRH-DE or a dominant negative isoform by microinjection of adeno-associated virus 1 (AAV1) vectors into the third ventr","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-05-23T03:14:05.174Z","creation":"2026-05-23T03:08:24.827Z"},"accession":"S-EPMC12110239","cross_references":{"pubmed":["40422228"],"doi":["10.3390/cells14100725"]}}