{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li P"],"funding":["Ligang Yuan","Youjun Shang"],"pagination":["529"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12111429"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(5)"],"pubmed_abstract":["<h4>Background</h4>In recent years, enzootic nasal tumor virus 2 (ENTV-2) has become prevalent in China, resulting in substantial economic losses for the goat industry. In order to enrich the availability of detection methods for ENTV-2, this study developed an expedited and accurate reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) assay to facilitate the detection and quantification of ENTV-2.<h4>Methods</h4>Specifically, a pair of primers and a TaqMan probe targeting conserved regions of the <i>pro</i> gene were designed to allow the specific amplification and detection of viral RNA in clinical samples. Moreover, modifying the method for use in a quantitative real-time PCR (qPCR) assay enables the detection of proviral DNA in tumor specimens.<h4>Results</h"],"journal":["Genes"],"pubmed_title":["Development and Application of a TaqMan-Based qPCR Assay for Detecting ENTV-2 in Goats."],"pmcid":["PMC12111429"],"funding_grant_id":["CARS-39-04B","2022YFD1602203","GSAU-JSFW-2023-07, GSAU-JSFW-2023-08","22CX8NA012","2024BBF02021"],"pubmed_authors":["Yin H","Wu J","Cao X","He J","Yuan L","Li P","Shang Y","Lan X"],"additional_accession":[]},"is_claimable":false,"name":"Development and Application of a TaqMan-Based qPCR Assay for Detecting ENTV-2 in Goats.","description":"<h4>Background</h4>In recent years, enzootic nasal tumor virus 2 (ENTV-2) has become prevalent in China, resulting in substantial economic losses for the goat industry. In order to enrich the availability of detection methods for ENTV-2, this study developed an expedited and accurate reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) assay to facilitate the detection and quantification of ENTV-2.<h4>Methods</h4>Specifically, a pair of primers and a TaqMan probe targeting conserved regions of the <i>pro</i> gene were designed to allow the specific amplification and detection of viral RNA in clinical samples. Moreover, modifying the method for use in a quantitative real-time PCR (qPCR) assay enables the detection of proviral DNA in tumor specimens.<h4>Results</h","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-04-23T03:20:33.954Z","creation":"2026-04-23T03:10:13.03Z"},"accession":"S-EPMC12111429","cross_references":{"pubmed":["40428351"],"doi":["10.3390/genes16050529"]}}