biostudies-literatureLi PLigang YuanYoujun Shang529https://www.ebi.ac.uk/biostudies/studies/S-EPMC12111429biostudies-literatureUnknown16(5)<h4>Background</h4>In recent years, enzootic nasal tumor virus 2 (ENTV-2) has become prevalent in China, resulting in substantial economic losses for the goat industry. In order to enrich the availability of detection methods for ENTV-2, this study developed an expedited and accurate reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) assay to facilitate the detection and quantification of ENTV-2.<h4>Methods</h4>Specifically, a pair of primers and a TaqMan probe targeting conserved regions of the <i>pro</i> gene were designed to allow the specific amplification and detection of viral RNA in clinical samples. Moreover, modifying the method for use in a quantitative real-time PCR (qPCR) assay enables the detection of proviral DNA in tumor specimens.<h4>Results</hGenesDevelopment and Application of a TaqMan-Based qPCR Assay for Detecting ENTV-2 in Goats.PMC12111429CARS-39-04B2022YFD1602203GSAU-JSFW-2023-07, GSAU-JSFW-2023-0822CX8NA0122024BBF02021Yin HWu JCao XHe JYuan LLi PShang YLan XfalseDevelopment and Application of a TaqMan-Based qPCR Assay for Detecting ENTV-2 in Goats.<h4>Background</h4>In recent years, enzootic nasal tumor virus 2 (ENTV-2) has become prevalent in China, resulting in substantial economic losses for the goat industry. In order to enrich the availability of detection methods for ENTV-2, this study developed an expedited and accurate reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) assay to facilitate the detection and quantification of ENTV-2.<h4>Methods</h4>Specifically, a pair of primers and a TaqMan probe targeting conserved regions of the <i>pro</i> gene were designed to allow the specific amplification and detection of viral RNA in clinical samples. Moreover, modifying the method for use in a quantitative real-time PCR (qPCR) assay enables the detection of proviral DNA in tumor specimens.<h4>Results</h2025-01-01T00:00:00Z2025 Apr2026-04-23T03:20:33.954Z2026-04-23T03:10:13.03ZS-EPMC121114294042835110.3390/genes16050529