{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["26(10)"],"submitter":["Kormann K"],"pubmed_abstract":["Immunoassays for cardiac troponin, such as the Elecsys<sup>®</sup> hs-TnT, have become the gold standard for myocardial infarction diagnostics. While various protein/chemical factors affecting the troponin complex and thus its diagnostic accuracy have been investigated, the role of coding single-nucleotide polymorphisms remains underexplored. To evaluate potential cSNP-induced interference with antibody binding in the Elecsys<sup>®</sup> hs-TnT immunoassay, we applied ITEM-FOUR, a mass spectrometry-based method that quantifies changes in antibody binding upon amino acid substitutions in epitope peptides. Candidate cSNPs were selected from the dbSNP database and were mapped to human cardiac troponin T by molecular modeling. Consuming micromolar antibody concentrations and microliter sample "],"journal":["International journal of molecular sciences"],"pagination":["4892"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12112476"],"repository":["biostudies-literature"],"pubmed_title":["Mass Spectrometric ITEM-FOUR Analysis Reveals Coding Single-Nucleotide Polymorphisms in Human Cardiac Troponin T That Evade Detection by Sandwich ELISAs Which Use Monoclonal Antibodies M7 and M11.7 from the Elecsys Troponin T&lt;sup&gt;®&lt;/sup&gt; Assay."],"pmcid":["PMC12112476"],"pubmed_authors":["Glocker MO","Ruß M","Kormann K","Koy C","Rower C"],"additional_accession":[]},"is_claimable":false,"name":"Mass Spectrometric ITEM-FOUR Analysis Reveals Coding Single-Nucleotide Polymorphisms in Human Cardiac Troponin T That Evade Detection by Sandwich ELISAs Which Use Monoclonal Antibodies M7 and M11.7 from the Elecsys Troponin T&lt;sup&gt;®&lt;/sup&gt; Assay.","description":"Immunoassays for cardiac troponin, such as the Elecsys<sup>®</sup> hs-TnT, have become the gold standard for myocardial infarction diagnostics. While various protein/chemical factors affecting the troponin complex and thus its diagnostic accuracy have been investigated, the role of coding single-nucleotide polymorphisms remains underexplored. To evaluate potential cSNP-induced interference with antibody binding in the Elecsys<sup>®</sup> hs-TnT immunoassay, we applied ITEM-FOUR, a mass spectrometry-based method that quantifies changes in antibody binding upon amino acid substitutions in epitope peptides. Candidate cSNPs were selected from the dbSNP database and were mapped to human cardiac troponin T by molecular modeling. Consuming micromolar antibody concentrations and microliter sample ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-05-23T03:12:46.913Z","creation":"2026-05-23T03:08:16.556Z"},"accession":"S-EPMC12112476","cross_references":{"pubmed":["40430031"],"doi":["10.3390/ijms26104892"]}}