{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang H"],"funding":["National Natural Science Foundation of China"],"pagination":["1121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12114444"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(5)"],"pubmed_abstract":["Two recombinant cutinases, <i>Af</i>CutA and <i>Af</i>CutB, derived from <i>Aspergillus fumigatus,</i> were heterologously expressed in <i>Pichia pastoris</i> and systematically characterized for their biochemical properties and polyester-degrading capabilities. <i>Af</i>CutA demonstrated superior catalytic performance compared with <i>Af</i>CutB, displaying higher optimal pH (8.0-9.0 vs. 7.0-8.0), higher optimal temperature (60 °C vs. 50 °C), and greater thermostability. <i>Af</i>CutA exhibited increased hydrolytic activity toward p-nitrophenyl esters (C4-C16) and synthetic polyesters. Additionally, <i>Af</i>CutA released approximately 3.2-fold more acetic acid from polyvinyl acetate (PVAc) hydrolysis than <i>Af</i>CutB. Quartz crystal microbalance with dissipation monitoring (QCM-D) reve"],"journal":["Microorganisms"],"pubmed_title":["Biochemical Characterization and Polyester-Binding/Degrading Capability of Two Cutinases from &lt;i&gt;Aspergillus fumigatus&lt;/i&gt;."],"pmcid":["PMC12114444"],"funding_grant_id":["22178179"],"pubmed_authors":["Zhang T","Chen K","Ding S","Long L","Wang H"],"additional_accession":[]},"is_claimable":false,"name":"Biochemical Characterization and Polyester-Binding/Degrading Capability of Two Cutinases from &lt;i&gt;Aspergillus fumigatus&lt;/i&gt;.","description":"Two recombinant cutinases, <i>Af</i>CutA and <i>Af</i>CutB, derived from <i>Aspergillus fumigatus,</i> were heterologously expressed in <i>Pichia pastoris</i> and systematically characterized for their biochemical properties and polyester-degrading capabilities. <i>Af</i>CutA demonstrated superior catalytic performance compared with <i>Af</i>CutB, displaying higher optimal pH (8.0-9.0 vs. 7.0-8.0), higher optimal temperature (60 °C vs. 50 °C), and greater thermostability. <i>Af</i>CutA exhibited increased hydrolytic activity toward p-nitrophenyl esters (C4-C16) and synthetic polyesters. Additionally, <i>Af</i>CutA released approximately 3.2-fold more acetic acid from polyvinyl acetate (PVAc) hydrolysis than <i>Af</i>CutB. Quartz crystal microbalance with dissipation monitoring (QCM-D) reve","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-04-23T03:19:50.19Z","creation":"2026-04-23T03:10:08.198Z"},"accession":"S-EPMC12114444","cross_references":{"pubmed":["40431293"],"doi":["10.3390/microorganisms13051121"]}}