{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Onuki Y"],"funding":["Japan Society for the Promotion of Science"],"pagination":["2101"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12114476"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30(10)"],"pubmed_abstract":["Malnutrition and aging are major factors that inhibit myoblast differentiation, leading to a decline in muscle function and contributing to sarcopenia development. This study aimed to elucidate the role of nutrients in myoblast differentiation by establishing a culture system at physiological glucose levels and investigating the effects of ketone bodies and oxaloacetate. We successfully cultured myoblasts at physiological glucose concentrations in a hydrophobic membrane filter-equipped culture flask. Under these conditions, ketone bodies and oxaloacetate synergistically upregulated myogenic differentiation markers (<i>Lmod2</i> and <i>Ckm</i>), indicating enhanced differentiation. Additionally, oxaloacetate upregulated mitochondrial biogenesis markers (mitochondrial DNA copy number and Cs)"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Oxaloacetate and Ketone Bodies Synergistically Promote Myoblast Differentiation in L6 Cells."],"pmcid":["PMC12114476"],"funding_grant_id":["JP23K10892"],"pubmed_authors":["Kojima-Yuasa A","Sasaki Y","Norikura T","Onuki Y","Nanashima N"],"additional_accession":[]},"is_claimable":false,"name":"Oxaloacetate and Ketone Bodies Synergistically Promote Myoblast Differentiation in L6 Cells.","description":"Malnutrition and aging are major factors that inhibit myoblast differentiation, leading to a decline in muscle function and contributing to sarcopenia development. This study aimed to elucidate the role of nutrients in myoblast differentiation by establishing a culture system at physiological glucose levels and investigating the effects of ketone bodies and oxaloacetate. We successfully cultured myoblasts at physiological glucose concentrations in a hydrophobic membrane filter-equipped culture flask. Under these conditions, ketone bodies and oxaloacetate synergistically upregulated myogenic differentiation markers (<i>Lmod2</i> and <i>Ckm</i>), indicating enhanced differentiation. Additionally, oxaloacetate upregulated mitochondrial biogenesis markers (mitochondrial DNA copy number and Cs)","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2025-07-26T03:07:01.478Z","creation":"2025-07-26T03:07:01.478Z"},"accession":"S-EPMC12114476","cross_references":{"pubmed":["40430274"],"doi":["10.3390/molecules30102101"]}}