biostudies-literatureKhan JHainan Province Science and Technology Special Fundthe National Parasitic Resources Center of ChinaKey R&D Plan Project of Hainan Provincethe Academician Innovation Platform Special Project of Hainan ProvinceMalaria R&D Sourcing & Facilitation in China201https://www.ebi.ac.uk/biostudies/studies/S-EPMC12128243biostudies-literatureUnknown18(1)<h4>Background</h4>Understanding the intrinsic factors that influence mosquito vector competence (VC) to pathogens is crucial for assessing the risk of disease transmission in both rural and urban environments. We assessed the VC of Aedes albopictus mosquitoes from urban (dengue-endemic) and rural (dengue-free) areas in Guangzhou, China, for dengue virus-2 (DENV-2), while also examining intrinsic factors such as Wolbachia and immune-related gene expression influencing VC.<h4>Methods</h4>Adult females of rural, urban, and laboratory (control) populations of Ae. albopictus were orally exposed to a freshly prepared suspension of the DENV-2 New Guinea C strain (GenBank: AF038403.1), with a final titer of 1 × 10⁷ plaque-forming units (PFU)/ml, for a period of 60 min. Three different Parasites & vectorsDynamics of vector competence for dengue virus type 2 in rural and urban populations of Aedes albopictus: implications for infectious disease control.PMC12128243ZDYF2021SHFZ091ZDKJ2021035 and ZDKJ202003NPRC-2019-194-30YSPTZX202133INV-061480Lv ZZhang DKhan JJunyan ZAdil MDeng YChen TfalseDynamics of vector competence for dengue virus type 2 in rural and urban populations of Aedes albopictus: implications for infectious disease control.<h4>Background</h4>Understanding the intrinsic factors that influence mosquito vector competence (VC) to pathogens is crucial for assessing the risk of disease transmission in both rural and urban environments. We assessed the VC of Aedes albopictus mosquitoes from urban (dengue-endemic) and rural (dengue-free) areas in Guangzhou, China, for dengue virus-2 (DENV-2), while also examining intrinsic factors such as Wolbachia and immune-related gene expression influencing VC.<h4>Methods</h4>Adult females of rural, urban, and laboratory (control) populations of Ae. albopictus were orally exposed to a freshly prepared suspension of the DENV-2 New Guinea C strain (GenBank: AF038403.1), with a final titer of 1 × 10⁷ plaque-forming units (PFU)/ml, for a period of 60 min. Three different 2025-01-01T00:00:00Z2025 Jun2026-06-02T00:14:51.379Z2026-05-24T03:07:44.146ZS-EPMC121282434045206210.1186/s13071-025-06826-8