{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hwang HJ"],"funding":["Henry M. Jackson Foundation","University of Iowa","National Institutes of Health","NIGMS NIH HHS","NIH HHS"],"pagination":["jcs263730"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12136168"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["138(9)"],"pubmed_abstract":["Drosophila Clu is a conserved multi-domain ribonucleoprotein essential for mitochondrial function that forms dynamic particles within the cytoplasm. Unlike stress granules and processing bodies (P-bodies), Clu particles disassemble under nutritional or oxidative stress. However, it is unclear how disrupting protein synthesis affects Clu particle dynamics, especially given that Clu binds mRNA and ribosomes. Here, we capitalize on ex vivo and in vivo imaging of Drosophila female germ cells to determine what domains of Clu are necessary for Clu particle assembly and how manipulating translation affects particle dynamics. Using domain deletion analysis, we identified three domains of Clu essential for particle assembly. We also demonstrated that overexpressing functional Clu led to disassembly"],"journal":["Journal of cell science"],"pubmed_title":["Drosophila Clu ribonucleoprotein particle dynamics rely on the availability of functional Clu and translating ribosomes."],"pmcid":["PMC12136168"],"funding_grant_id":["GM127938","R01 GM127938","1R01GM127938","P40 OD018537"],"pubmed_authors":["Cox RT","Sheard KM","Hwang HJ"],"additional_accession":[]},"is_claimable":false,"name":"Drosophila Clu ribonucleoprotein particle dynamics rely on the availability of functional Clu and translating ribosomes.","description":"Drosophila Clu is a conserved multi-domain ribonucleoprotein essential for mitochondrial function that forms dynamic particles within the cytoplasm. Unlike stress granules and processing bodies (P-bodies), Clu particles disassemble under nutritional or oxidative stress. However, it is unclear how disrupting protein synthesis affects Clu particle dynamics, especially given that Clu binds mRNA and ribosomes. Here, we capitalize on ex vivo and in vivo imaging of Drosophila female germ cells to determine what domains of Clu are necessary for Clu particle assembly and how manipulating translation affects particle dynamics. Using domain deletion analysis, we identified three domains of Clu essential for particle assembly. We also demonstrated that overexpressing functional Clu led to disassembly","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-07-15T12:36:50.056Z","creation":"2026-07-04T03:14:13.969Z"},"accession":"S-EPMC12136168","cross_references":{"pubmed":["40302698"],"doi":["10.1242/jcs.263730"]}}