biostudies-literatureBui LDMinistry of Science and Technology, Taiwan137https://www.ebi.ac.uk/biostudies/studies/S-EPMC12137402biostudies-literatureUnknown109(1)Pseudomonas nitroreducens ATCC PTA-6168 (also named TX1) efficiently grows on non-ionic surfactants as the sole source of carbon and energy aerobically. The global gene expression and bacterial stress responses during degradation remain further investigated. This study compared the growth, viability, and transcriptomic profiles of strain TX1 during the log phase when grown on minimal salts basal medium supplemented with 0.5% octylphenol polyethoxylates (OPEO_n, commercial name Triton^® X-100) vs. in 0.5% succinate. Differentially expressed genes were identified, with 219 upregulated and 22 downregulated. Gene ontology analysis revealed upregulation of oxidoreductase activity (53%), electrotransfer activity (11%), heme/FAD/cofactor binding (11/19/27%), and membrane (42%)Applied microbiology and biotechnologyGrowth and gene expression of Pseudomonas nitroreducens TX1 on octylphenol polyethoxylate surfactants.PMC12137402MOST109-2320-B-010-010-MY3MOST106-2320-B-010-020-MY3Bui LDTsai PCChen CCNguyen TNHuang SLfalseGrowth and gene expression of Pseudomonas nitroreducens TX1 on octylphenol polyethoxylate surfactants.Pseudomonas nitroreducens ATCC PTA-6168 (also named TX1) efficiently grows on non-ionic surfactants as the sole source of carbon and energy aerobically. The global gene expression and bacterial stress responses during degradation remain further investigated. This study compared the growth, viability, and transcriptomic profiles of strain TX1 during the log phase when grown on minimal salts basal medium supplemented with 0.5% octylphenol polyethoxylates (OPEO_n, commercial name Triton^® X-100) vs. in 0.5% succinate. Differentially expressed genes were identified, with 219 upregulated and 22 downregulated. Gene ontology analysis revealed upregulation of oxidoreductase activity (53%), electrotransfer activity (11%), heme/FAD/cofactor binding (11/19/27%), and membrane (42%)2025-01-01T00:00:00Z2025 Jun2026-06-15T06:11:40.564Z2026-06-15T03:08:27.562ZS-EPMC121374024046805910.1007/s00253-025-13463-9