{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Martianov I"],"funding":["National League Against Cancer","French National Research Agency"],"pagination":["112286"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12148609"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(6)"],"pubmed_abstract":["The multiprotein complex TFIID, comprising the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is an essential component of the RNA polymerase II (Pol II) preinitiation complex (PIC). Cryo-electron microscopy studies suggested a critical role of the TAF11-TAF13 heterodimer in TBP promoter deposition upstream of the transcription start site. To investigate this hypothesis, we inactivated the gene encoding Taf13 in mice and embryonic stem cells (ESCs). Taf13-null embryos implant and survive until E6.5, but fail to undergo gastrulation, while Taf13-null ESCs are viable, but fail to form embryoid bodies and differentiate. Taf13 loss had little effect on TFIID integrity and led to only a mild reduction of TBP promoter recruitment, but led to altered PIC formation and globally r"],"journal":["iScience"],"pubmed_title":["The transcription factor IID subunit Taf13 is dispensable for TATA binding protein promoter recruitment and RNA polymerase II transcription."],"pmcid":["PMC12148609"],"funding_grant_id":["ANR10-INBS-09-08","ANR-10-LABX-0030-INRT","ANR-10-INBS-04"],"pubmed_authors":["Davidson I","Davidson G","Martianov I","Le Gras S"],"additional_accession":[]},"is_claimable":false,"name":"The transcription factor IID subunit Taf13 is dispensable for TATA binding protein promoter recruitment and RNA polymerase II transcription.","description":"The multiprotein complex TFIID, comprising the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is an essential component of the RNA polymerase II (Pol II) preinitiation complex (PIC). Cryo-electron microscopy studies suggested a critical role of the TAF11-TAF13 heterodimer in TBP promoter deposition upstream of the transcription start site. To investigate this hypothesis, we inactivated the gene encoding Taf13 in mice and embryonic stem cells (ESCs). Taf13-null embryos implant and survive until E6.5, but fail to undergo gastrulation, while Taf13-null ESCs are viable, but fail to form embryoid bodies and differentiate. Taf13 loss had little effect on TFIID integrity and led to only a mild reduction of TBP promoter recruitment, but led to altered PIC formation and globally r","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-06-01T15:41:48.651Z","creation":"2026-04-08T13:46:36.801Z"},"accession":"S-EPMC12148609","cross_references":{"pubmed":["40491483"],"doi":["10.1016/j.isci.2025.112286"]}}