biostudies-literatureUnknown16Griesbaum L<h4>Introduction</h4>As a part of the innate immune system, eosinophils are recruited during infectious diseases, to release their characteristic cytotoxic granules and catch pathogens in extracellular traps. Moreover, eosinophils have a crucial role in autoimmune diseases, for example allergies. The isolation of these densest and lowest abundant leukocytes is cost-and labor intense. This sets restrictions on many aspects of eosinophilic research. In this study, we performed a thorough characterization and functional assessment of the HL-60 clone 15 (HC15) cell line, which can be differentiated into eosinophil-like cells, to investigate its potential in eosinophil research.<h4>Methods</h4>HC15 cells were differentiated with sodium butyrate with or without IL-5 and cells were characterized Frontiers in immunology1515993https://www.ebi.ac.uk/biostudies/studies/S-EPMC12148902biostudies-literatureThe HL-60 clone 15 cell line as a model for leukocyte migration-possibilities and limitations.PMC12148902Griesbaum LWeusthof CKlimek LSauer TGemoll TPetry JBashiri Dezfouli AShoykhet MWeirich GWollenberg BfalseThe HL-60 clone 15 cell line as a model for leukocyte migration-possibilities and limitations.<h4>Introduction</h4>As a part of the innate immune system, eosinophils are recruited during infectious diseases, to release their characteristic cytotoxic granules and catch pathogens in extracellular traps. Moreover, eosinophils have a crucial role in autoimmune diseases, for example allergies. The isolation of these densest and lowest abundant leukocytes is cost-and labor intense. This sets restrictions on many aspects of eosinophilic research. In this study, we performed a thorough characterization and functional assessment of the HL-60 clone 15 (HC15) cell line, which can be differentiated into eosinophil-like cells, to investigate its potential in eosinophil research.<h4>Methods</h4>HC15 cells were differentiated with sodium butyrate with or without IL-5 and cells were characterized 2025-01-01T00:00:00Z20252026-06-02T20:31:32.469Z2026-04-20T03:10:19.136ZS-EPMC121489024049686510.3389/fimmu.2025.1515993