{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chacon J"],"funding":["Ministerio de Ciencia y Tecnolog?a","Vicerrector?a de Investigaci?n, Universidad de Costa Rica"],"pagination":["2801-2815"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12150329"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(6)"],"pubmed_abstract":["We report the biochemical and proteomic characterization of the venom of the arboreal Talamancan palm-pitviper, <i>Bothriechis nubestris</i>, a species endemic to north and central parts of the Costa Rican Cordillera de Talamanca, at 2100 to 3000 m above sea level. The Talamancan palm-pitviper venom arsenal comprised the translated products of 26 unique transcripts into 10 toxin classes, where metalloproteinases PIIa and PIII represent the dominant components. <i>In vitro</i>, the venom proteolyzed azocasein and gelatin but showed no phospholipase A<sub>2</sub> or human plasma coagulant activities. <i>In vivo</i>, <i>B. nubestris</i> venom exhibited an intravenous median lethal dose (LD<sub>50</sub>) of 21.5 (95% confidence interval: 15.6-29.5) μg/mouse, and a minimum hemorrhagic dose (MHD"],"journal":["Journal of proteome research"],"pubmed_title":["Snake Venomics of the Arboreal Talamancan Palm-Pitviper, &lt;i&gt;Bothriechis nubestris&lt;/i&gt;, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms."],"pmcid":["PMC12150329"],"funding_grant_id":["C0061","BFU2020_PID2020-119593GB-I00"],"pubmed_authors":["Chacon J","Lomonte B","Fernandez J","Mena G","Sasa M","Bonilla F","Sanz L","Chang-Castillo A","Gutierrez JM","Calvete JJ","Diaz C","Chaves-Araya S"],"additional_accession":[]},"is_claimable":false,"name":"Snake Venomics of the Arboreal Talamancan Palm-Pitviper, &lt;i&gt;Bothriechis nubestris&lt;/i&gt;, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms.","description":"We report the biochemical and proteomic characterization of the venom of the arboreal Talamancan palm-pitviper, <i>Bothriechis nubestris</i>, a species endemic to north and central parts of the Costa Rican Cordillera de Talamanca, at 2100 to 3000 m above sea level. The Talamancan palm-pitviper venom arsenal comprised the translated products of 26 unique transcripts into 10 toxin classes, where metalloproteinases PIIa and PIII represent the dominant components. <i>In vitro</i>, the venom proteolyzed azocasein and gelatin but showed no phospholipase A<sub>2</sub> or human plasma coagulant activities. <i>In vivo</i>, <i>B. nubestris</i> venom exhibited an intravenous median lethal dose (LD<sub>50</sub>) of 21.5 (95% confidence interval: 15.6-29.5) μg/mouse, and a minimum hemorrhagic dose (MHD","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-07-15T06:29:07.749Z","creation":"2026-06-30T03:21:37.439Z"},"accession":"S-EPMC12150329","cross_references":{"pubmed":["40277170"],"doi":["10.1021/acs.jproteome.4c01041"]}}