{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["134(9)"],"submitter":["Gao Q"],"pubmed_abstract":["<h4>Aims</h4>To investigate the most suitable housekeeping genes for quantifying a change in mRNA expression levels due to hepatitis virus B related hepatocellular carcinoma (HCC).<h4>Methods</h4>Expression of mRNA encoding ACTB, GAPDH, B2M, HPRT and TBP was measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in matched malignant and non-malignant tissues obtained from 65 non-treated HCCs. The software programs geNorm and NormFinder were used to ascertain the most suitable reference gene combination.<h4>Results</h4>All candidate genes showed significantly different expression between malignant and non-malignant samples. GAPDH and ACTB, genes most frequently used for normalization, were heavily regulated during HCC carcinogenesis and progression. B2M expres"],"journal":["Journal of cancer research and clinical oncology"],"pagination":["979-86"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12160763"],"repository":["biostudies-literature"],"pubmed_title":["Selection of reference genes for real-time PCR in human hepatocellular carcinoma tissues."],"pmcid":["PMC12160763"],"pubmed_authors":["Gao Q","Xiao YS","Huang XW","Fan J","Shi YH","Sun J","Wang XY","Qiu SJ","Zhou J","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Selection of reference genes for real-time PCR in human hepatocellular carcinoma tissues.","description":"<h4>Aims</h4>To investigate the most suitable housekeeping genes for quantifying a change in mRNA expression levels due to hepatitis virus B related hepatocellular carcinoma (HCC).<h4>Methods</h4>Expression of mRNA encoding ACTB, GAPDH, B2M, HPRT and TBP was measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in matched malignant and non-malignant tissues obtained from 65 non-treated HCCs. The software programs geNorm and NormFinder were used to ascertain the most suitable reference gene combination.<h4>Results</h4>All candidate genes showed significantly different expression between malignant and non-malignant samples. GAPDH and ACTB, genes most frequently used for normalization, were heavily regulated during HCC carcinogenesis and progression. B2M expres","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Sep","modification":"2026-03-27T15:21:22.82Z","creation":"2025-08-27T03:07:16.418Z"},"accession":"S-EPMC12160763","cross_references":{"pubmed":["18317805"],"doi":["10.1007/s00432-008-0369-3"]}}