biostudies-literatureUnknown134(9)Gao Q<h4>Aims</h4>To investigate the most suitable housekeeping genes for quantifying a change in mRNA expression levels due to hepatitis virus B related hepatocellular carcinoma (HCC).<h4>Methods</h4>Expression of mRNA encoding ACTB, GAPDH, B2M, HPRT and TBP was measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in matched malignant and non-malignant tissues obtained from 65 non-treated HCCs. The software programs geNorm and NormFinder were used to ascertain the most suitable reference gene combination.<h4>Results</h4>All candidate genes showed significantly different expression between malignant and non-malignant samples. GAPDH and ACTB, genes most frequently used for normalization, were heavily regulated during HCC carcinogenesis and progression. B2M expresJournal of cancer research and clinical oncology979-86https://www.ebi.ac.uk/biostudies/studies/S-EPMC12160763biostudies-literatureSelection of reference genes for real-time PCR in human hepatocellular carcinoma tissues.PMC12160763Gao QXiao YSHuang XWFan JShi YHSun JWang XYQiu SJZhou JXu YfalseSelection of reference genes for real-time PCR in human hepatocellular carcinoma tissues.<h4>Aims</h4>To investigate the most suitable housekeeping genes for quantifying a change in mRNA expression levels due to hepatitis virus B related hepatocellular carcinoma (HCC).<h4>Methods</h4>Expression of mRNA encoding ACTB, GAPDH, B2M, HPRT and TBP was measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in matched malignant and non-malignant tissues obtained from 65 non-treated HCCs. The software programs geNorm and NormFinder were used to ascertain the most suitable reference gene combination.<h4>Results</h4>All candidate genes showed significantly different expression between malignant and non-malignant samples. GAPDH and ACTB, genes most frequently used for normalization, were heavily regulated during HCC carcinogenesis and progression. B2M expres2008-01-01T00:00:00Z2008 Sep2026-03-27T15:21:22.82Z2025-08-27T03:07:16.418ZS-EPMC121607631831780510.1007/s00432-008-0369-3