biostudies-literatureUnknown16Lonardi S<h4>Background</h4>The retinoic-acid-receptor-related orphan receptor gamma t (RORγt) isoform is required for the development of lymphoid organs, T-helper 17 cells (Th17), and innate lymphoid cells (ILC3s). Recent data in mouse and human have revealed non-T, non-ILC3 cell populations with antigen presenting features that express RORγt. This study maps the presence of RORγt cells with dendritic cell (DC) features in human adult and fetal lymphoid tissues.<h4>Methods</h4>By combining multicolor flow cytometry, RNAseq of peripheral blood cells, analysis of lymph node scRNAseq datasets, and microscopic analysis on human tissue sections and single-cell suspensions, this study maps the presence of RORγt cells with DC features in human tissues.<h4>Results</h4>RORγt-DCs are found in human lymphoidFrontiers in immunology1527499https://www.ebi.ac.uk/biostudies/studies/S-EPMC12162286biostudies-literatureMapping of RORγt&lt;sup&gt;+&lt;/sup&gt; dendritic cells in human tissues establishes their preferential niche in adult lymph nodes.PMC12162286Bianchetto-Aguilera FMMonti MMissale FBugatti MAntonova AULonardi SFerrari GColonna MLorenzi LSavoldi VCassatella MADi Matteo AZini SGhigna CCella MBenedetti MFacchetti FWu SAgostini VPicinoli SVermi WChiarini MLiu YAMoratto DfalseMapping of RORγt&lt;sup&gt;+&lt;/sup&gt; dendritic cells in human tissues establishes their preferential niche in adult lymph nodes.<h4>Background</h4>The retinoic-acid-receptor-related orphan receptor gamma t (RORγt) isoform is required for the development of lymphoid organs, T-helper 17 cells (Th17), and innate lymphoid cells (ILC3s). Recent data in mouse and human have revealed non-T, non-ILC3 cell populations with antigen presenting features that express RORγt. This study maps the presence of RORγt cells with dendritic cell (DC) features in human adult and fetal lymphoid tissues.<h4>Methods</h4>By combining multicolor flow cytometry, RNAseq of peripheral blood cells, analysis of lymph node scRNAseq datasets, and microscopic analysis on human tissue sections and single-cell suspensions, this study maps the presence of RORγt cells with DC features in human tissues.<h4>Results</h4>RORγt-DCs are found in human lymphoid2025-01-01T00:00:00Z20252026-05-26T20:10:27.086Z2026-05-26T03:07:13.38ZS-EPMC121622864051991310.3389/fimmu.2025.1527499