biostudies-literatureUnknown16Kumar RRThiol-based redox sensing has been found to play diverse roles in regulating various metabolic pathways. Here, the thiol-based redox system of 16 diverse genotypes of wheat grains was characterized, and it correlated with the accumulation of macro-/micronutrients inside the grains. We observed significant variations in the thiol and disulfide content in the grains. An expression analysis of the genes responsible for thiol-based redox sensing, such as thioredoxin (<i>TRX</i>), glutaredoxin (<i>GRX</i>) and glutathione reductase (<i>GR</i>), showed maximum fold expression in wheat <i>cvs</i>. Halna and HD2985 (high thiol <i>cvs</i>.) during the seed hardening stage (G<sub>2</sub>) of endosperm, as compared to low thiol-containing <i>cvs</i>. We retrieved the amino acid sequences of 11 genes Frontiers in plant science1488697https://www.ebi.ac.uk/biostudies/studies/S-EPMC12162979biostudies-literatureThiol-based redox sensing regulates the yellow pigment and antioxidant accumulation and improves the nutritional quality of wheat grains (<i>Triticum aestivum</i> L.).PMC12162979Goswami SMishra GPGampa MT VJha GKBabu H PKumar SKumar RRMishra DTyagi APraveen SC VKumar SNHasija SRai GKfalseThiol-based redox sensing regulates the yellow pigment and antioxidant accumulation and improves the nutritional quality of wheat grains (<i>Triticum aestivum</i> L.).Thiol-based redox sensing has been found to play diverse roles in regulating various metabolic pathways. Here, the thiol-based redox system of 16 diverse genotypes of wheat grains was characterized, and it correlated with the accumulation of macro-/micronutrients inside the grains. We observed significant variations in the thiol and disulfide content in the grains. An expression analysis of the genes responsible for thiol-based redox sensing, such as thioredoxin (<i>TRX</i>), glutaredoxin (<i>GRX</i>) and glutathione reductase (<i>GR</i>), showed maximum fold expression in wheat <i>cvs</i>. Halna and HD2985 (high thiol <i>cvs</i>.) during the seed hardening stage (G<sub>2</sub>) of endosperm, as compared to low thiol-containing <i>cvs</i>. We retrieved the amino acid sequences of 11 genes 2025-01-01T00:00:00Z20252026-06-01T23:20:10.109Z2026-05-23T03:08:06.77ZS-EPMC121629794051959210.3389/fpls.2025.1488697