{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen C"],"funding":["Institute for Digital Molecular Analytics and Science, Nanyang Technological University","Dutch Research Council (NWO)","VLAG Graduate School","Division of Materials Research","Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University"],"pagination":["20550-20563"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12164530"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(22)"],"pubmed_abstract":["Living cells orchestrate a myriad of biological reactions within a highly complex and crowded environment. A major factor responsible for such seamless assembly is the preferential interactions between the constituent macromolecules, that can drive demixing to produce coexisting phases and thus provide dynamic intracellular compartmentalization. However, the way multiple-phase separation phenomena, occurring simultaneously within the cytoplasmic space, influence each other is still largely unknown. Here, we show that the interplay between segregative and associative phase separation within cell-mimicking confinements can lead to rich dynamics between multiple phases and the lipid boundary. Using on-chip microfluidic systems, we encapsulate the associative and segregative components and ext"],"journal":["ACS nano"],"pubmed_title":["Regulating Biocondensates within Synthetic Cells via Segregative Phase Separation."],"pmcid":["PMC12164530"],"funding_grant_id":["DMR-2342436","OCENW.KLEIN.465"],"pubmed_authors":["Ganar KA","Carnahan CF","Parikh AN","Deshpande S","Love CM","Chen C"],"additional_accession":[]},"is_claimable":false,"name":"Regulating Biocondensates within Synthetic Cells via Segregative Phase Separation.","description":"Living cells orchestrate a myriad of biological reactions within a highly complex and crowded environment. A major factor responsible for such seamless assembly is the preferential interactions between the constituent macromolecules, that can drive demixing to produce coexisting phases and thus provide dynamic intracellular compartmentalization. However, the way multiple-phase separation phenomena, occurring simultaneously within the cytoplasmic space, influence each other is still largely unknown. Here, we show that the interplay between segregative and associative phase separation within cell-mimicking confinements can lead to rich dynamics between multiple phases and the lipid boundary. Using on-chip microfluidic systems, we encapsulate the associative and segregative components and ext","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-07-15T05:58:01.268Z","creation":"2026-06-30T03:23:03.874Z"},"accession":"S-EPMC12164530","cross_references":{"pubmed":["40293809"],"doi":["10.1021/acsnano.4c18971"]}}