{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(1)"],"submitter":["Daum AK"],"pubmed_abstract":["<h4>Background</h4>Targeted therapy interventions using tyrosine kinase inhibitors (TKIs) provide encouraging treatment responses in patients with ALK-rearranged lung adenocarcinomas, yet resistance occurs almost inevitably. In addition to tumor cell-intrinsic resistance mechanisms, accumulating evidence suggests that cancer-associated fibroblasts (CAFs) within the tumor microenvironment contribute to therapy resistance. This study aimed to investigate CAF-driven molecular networks that shape the therapeutic susceptibility of ALK-driven lung adenocarcinoma cells.<h4>Methods</h4>Three-dimensional (3D) spheroid co-cultures comprising ALK-rearranged lung adenocarcinoma cells and CAFs were utilized to model the tumor microenvironment. Single-cell RNA sequencing was performed to uncover transcr"],"journal":["Cancer & metabolism"],"pagination":["28"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12168422"],"repository":["biostudies-literature"],"pubmed_title":["Cancer-associated fibroblasts promote drug resistance in ALK-driven lung adenocarcinoma cells by upregulating lipid biosynthesis."],"pmcid":["PMC12168422"],"pubmed_authors":["Thomas M","Muley T","Klingmuller U","Sultmann H","Daum AK","Schulze A","Schneider MA","Schlicker L","Christopoulos P"],"additional_accession":[]},"is_claimable":false,"name":"Cancer-associated fibroblasts promote drug resistance in ALK-driven lung adenocarcinoma cells by upregulating lipid biosynthesis.","description":"<h4>Background</h4>Targeted therapy interventions using tyrosine kinase inhibitors (TKIs) provide encouraging treatment responses in patients with ALK-rearranged lung adenocarcinomas, yet resistance occurs almost inevitably. In addition to tumor cell-intrinsic resistance mechanisms, accumulating evidence suggests that cancer-associated fibroblasts (CAFs) within the tumor microenvironment contribute to therapy resistance. This study aimed to investigate CAF-driven molecular networks that shape the therapeutic susceptibility of ALK-driven lung adenocarcinoma cells.<h4>Methods</h4>Three-dimensional (3D) spheroid co-cultures comprising ALK-rearranged lung adenocarcinoma cells and CAFs were utilized to model the tumor microenvironment. Single-cell RNA sequencing was performed to uncover transcr","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-06-02T22:38:15.722Z","creation":"2026-04-21T03:13:47.899Z"},"accession":"S-EPMC12168422","cross_references":{"pubmed":["40524253"],"doi":["10.1186/s40170-025-00400-7"]}}