biostudies-literatureUnknown20Shoaib MExtended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> resists broad-spectrum cephalosporins, considered as a critical priority pathogen, and its presence in animals, humans, and the environment highlights its significance as a One Health issue. Dairy farm waste is a potential environmental contaminant and can serves as a significant reservoir for the emergence and spread of ESBL-producing <i>E. coli</i>, which belongs to One Health clones and poses a serious global threat. This study aimed to investigate the occurrence and genomic characteristics of ESBL-producing <i>E. coli</i> clones of One Health concern from dairy farm waste in Gansu, China. In this study, we isolated and characterized two CTX-M-55 ESBL-producing <i>E. coli</i> strains, ZYX8158 and ZYS8091, which beOne health (Amsterdam, Netherlands)101101https://www.ebi.ac.uk/biostudies/studies/S-EPMC12179727biostudies-literatureGenomic and phylogeographical analysis revealed CTX-M-55 producing <i>Escherichia coli</i> ST10 and ST2325 clones of One Health concern from dairy farm waste in Gansu, China.PMC12179727Wu ZTang MWeiwei WHe ZShoaib MWang SZhang XPu WLi RHao BAli MMfalseGenomic and phylogeographical analysis revealed CTX-M-55 producing <i>Escherichia coli</i> ST10 and ST2325 clones of One Health concern from dairy farm waste in Gansu, China.Extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> resists broad-spectrum cephalosporins, considered as a critical priority pathogen, and its presence in animals, humans, and the environment highlights its significance as a One Health issue. Dairy farm waste is a potential environmental contaminant and can serves as a significant reservoir for the emergence and spread of ESBL-producing <i>E. coli</i>, which belongs to One Health clones and poses a serious global threat. This study aimed to investigate the occurrence and genomic characteristics of ESBL-producing <i>E. coli</i> clones of One Health concern from dairy farm waste in Gansu, China. In this study, we isolated and characterized two CTX-M-55 ESBL-producing <i>E. coli</i> strains, ZYX8158 and ZYS8091, which be2025-01-01T00:00:00Z2025 Jun2026-04-08T18:47:53.974Z2026-04-08T10:45:28.087ZS-EPMC121797274054720810.1016/j.onehlt.2025.101101