biostudies-literatureUnknown15(1)Aboul-Nasr MBSohag UniversityPaclitaxel (PTX) is a potent chemotherapeutic agent limited by poor solubility and adverse effects. To address these challenges, we developed a novel nanocomposite combining PTX purified from the endophytic fungus A. fumigatiaffinis PP235788.1 with biosynthesized silver nanoparticles (AgNPs) from the same fungal strain. The PTX-AgNP conjugate (28.48 ± 0.13 nm) was characterized by UV-Vis, XRD, and TEM, revealing monodisperse AgNPs (14.50 ± 0.58 nm) as the core component. In vitro studies demonstrated significant cytotoxicity against MCF-7 breast cancer cells (IC₅₀= 1.7 µg/mL, p < 0.001), with 5-10-fold greater efficacy than AgNPs alone. Annexin V/PI staining and DNA fragmentation assays confirmed apoptosis induction, highlighting the conjugate's enhanced anticancer activity. This eco-frienScientific reports20198https://www.ebi.ac.uk/biostudies/studies/S-EPMC12181376biostudies-literatureExploring the anticancer potential of green silver Nanoparticles-Paclitaxel nanocarrier on MCF-7 breast Cancer cells: an in vitro approach.PMC12181376Aboul-Nasr MBAboul-Nasr YBMohamed SSObiedallah MYasien AAfalseExploring the anticancer potential of green silver Nanoparticles-Paclitaxel nanocarrier on MCF-7 breast Cancer cells: an in vitro approach.Paclitaxel (PTX) is a potent chemotherapeutic agent limited by poor solubility and adverse effects. To address these challenges, we developed a novel nanocomposite combining PTX purified from the endophytic fungus A. fumigatiaffinis PP235788.1 with biosynthesized silver nanoparticles (AgNPs) from the same fungal strain. The PTX-AgNP conjugate (28.48 ± 0.13 nm) was characterized by UV-Vis, XRD, and TEM, revealing monodisperse AgNPs (14.50 ± 0.58 nm) as the core component. In vitro studies demonstrated significant cytotoxicity against MCF-7 breast cancer cells (IC₅₀= 1.7 µg/mL, p < 0.001), with 5-10-fold greater efficacy than AgNPs alone. Annexin V/PI staining and DNA fragmentation assays confirmed apoptosis induction, highlighting the conjugate's enhanced anticancer activity. This eco-frien2025-01-01T00:00:00Z2025 Jun2025-07-25T03:08:12.82Z2025-07-25T03:08:12.82ZS-EPMC121813764054211510.1038/s41598-025-06275-4