{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang J"],"funding":["Youth project of Wuxi commission of Health","National Natural Science Foundation of China","Wuxi Taihu Lake Talent Plan","Scientific Research Project of Wuxi Health Commission"],"pagination":["2518679"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12184166"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Glioblastoma (GBM) exhibits elevated TRIM22 expression correlated with tumor progression, as validated in TCGA/GEO databases. The effects of TRIM22 knockdown and overexpression on GBM proliferation were evaluated with cellular assays. TRIM22 was identified as a potential Bcl-2 activator via a ubiquitination microarray. Flow cytometry (FCM) was utilized to investigate cell apoptosis. Additionally, the expression levels of Bcl-2 and proteins associated with Bcl-2 were evaluated using Western blot analysis. The interaction and ubiquitination of TRIM22 and Bcl-2 were analyzed via immunoprecipitation (IP). TRIM22 overexpression is correlated with glioma progression, and TRIM22 deficiency inhibits GBM cell proliferation. FCM revealed that TRIM22 knockdown promotes GBM cell apoptosis. A TRIM22-ov"],"journal":["Molecular & cellular oncology"],"pubmed_title":["TRIM22 promotes glioblastoma development by ubiquitinating Bcl-2."],"pmcid":["PMC12184166"],"funding_grant_id":["82172955","Q202133","2020ZHYB16","DJYX-2020"],"pubmed_authors":["Cheng C","Liu Y","Shao J","Zhang J","Huang J","Huang H","Chen Y","Wang G","Jiao J"],"additional_accession":[]},"is_claimable":false,"name":"TRIM22 promotes glioblastoma development by ubiquitinating Bcl-2.","description":"Glioblastoma (GBM) exhibits elevated TRIM22 expression correlated with tumor progression, as validated in TCGA/GEO databases. The effects of TRIM22 knockdown and overexpression on GBM proliferation were evaluated with cellular assays. TRIM22 was identified as a potential Bcl-2 activator via a ubiquitination microarray. Flow cytometry (FCM) was utilized to investigate cell apoptosis. Additionally, the expression levels of Bcl-2 and proteins associated with Bcl-2 were evaluated using Western blot analysis. The interaction and ubiquitination of TRIM22 and Bcl-2 were analyzed via immunoprecipitation (IP). TRIM22 overexpression is correlated with glioma progression, and TRIM22 deficiency inhibits GBM cell proliferation. FCM revealed that TRIM22 knockdown promotes GBM cell apoptosis. A TRIM22-ov","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-03T07:03:38.175Z","creation":"2026-04-25T03:21:58.598Z"},"accession":"S-EPMC12184166","cross_references":{"pubmed":["40552115"],"doi":["10.1080/23723556.2025.2518679"]}}