biostudies-literatureZhang JYouth project of Wuxi commission of HealthNational Natural Science Foundation of ChinaWuxi Taihu Lake Talent PlanScientific Research Project of Wuxi Health Commission2518679https://www.ebi.ac.uk/biostudies/studies/S-EPMC12184166biostudies-literatureUnknown12(1)Glioblastoma (GBM) exhibits elevated TRIM22 expression correlated with tumor progression, as validated in TCGA/GEO databases. The effects of TRIM22 knockdown and overexpression on GBM proliferation were evaluated with cellular assays. TRIM22 was identified as a potential Bcl-2 activator via a ubiquitination microarray. Flow cytometry (FCM) was utilized to investigate cell apoptosis. Additionally, the expression levels of Bcl-2 and proteins associated with Bcl-2 were evaluated using Western blot analysis. The interaction and ubiquitination of TRIM22 and Bcl-2 were analyzed via immunoprecipitation (IP). TRIM22 overexpression is correlated with glioma progression, and TRIM22 deficiency inhibits GBM cell proliferation. FCM revealed that TRIM22 knockdown promotes GBM cell apoptosis. A TRIM22-ovMolecular & cellular oncologyTRIM22 promotes glioblastoma development by ubiquitinating Bcl-2.PMC1218416682172955Q2021332020ZHYB16DJYX-2020Cheng CLiu YShao JZhang JHuang JHuang HChen YWang GJiao JfalseTRIM22 promotes glioblastoma development by ubiquitinating Bcl-2.Glioblastoma (GBM) exhibits elevated TRIM22 expression correlated with tumor progression, as validated in TCGA/GEO databases. The effects of TRIM22 knockdown and overexpression on GBM proliferation were evaluated with cellular assays. TRIM22 was identified as a potential Bcl-2 activator via a ubiquitination microarray. Flow cytometry (FCM) was utilized to investigate cell apoptosis. Additionally, the expression levels of Bcl-2 and proteins associated with Bcl-2 were evaluated using Western blot analysis. The interaction and ubiquitination of TRIM22 and Bcl-2 were analyzed via immunoprecipitation (IP). TRIM22 overexpression is correlated with glioma progression, and TRIM22 deficiency inhibits GBM cell proliferation. FCM revealed that TRIM22 knockdown promotes GBM cell apoptosis. A TRIM22-ov2025-01-01T00:00:00Z20252026-06-03T07:03:38.175Z2026-04-25T03:21:58.598ZS-EPMC121841664055211510.1080/23723556.2025.2518679