{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Toomey CB"],"funding":["NEI NIH HHS","International Retinal Research Foundation (IRRF)","Foundation Fighting Blindness (FFB)","HHS | NIH | National Eye Institute (NEI)","NIAID NIH HHS","NHLBI NIH HHS","Novartis AG | Alcon | Alcon Research Institute (ARI)","HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI)","Research to Prevent Blindness (RPB)","Larry L. Hillblom Foundation (LLHF)"],"pagination":["e2500727122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12184332"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["122(24)"],"pubmed_abstract":["Lipoprotein retention in Bruch's membrane is a key event in the pathobiology of early and intermediate age-related macular degeneration (AMD). However, the mechanism of lipoprotein retention in BrM is unknown. Given the established role of glycosaminoglycans (GAG) in binding lipoproteins, our laboratory sought to determine the role of GAGs in AMD BrM. In this study, BrM GAG content in AMD pathobiology was analyzed in human postmortem tissue. Strikingly, increased levels of highly sulfated heparan sulfate were present in AMD Bruch's membrane as compared to non-AMD samples. In addition, using scanning electron microscopy of postmortem AMD tissue, we show aggregates of lipoprotein-like particles on the retinal pigmented epithelium side of Bruch's membrane adjacent to heparan sulfate. We also "],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Bruch's membrane heparan sulfate retains lipoproteins in the early stages of age-related macular degeneration."],"pmcid":["PMC12184332"],"funding_grant_id":["R01 EY030501","P01 HL131474","EY024225","R21 AI185519","R01 EY031594","RPB CDA 2023","A-013","RMF-IRRF-001-2022","EY033765","unrestricted grant","EY030501","EY035805","Unrestricted Grant","K08 EY035322","HL131474","R01 EY033765","CD-CL-0824-0886-UCSD","EY031594","R01 EY035805","EY035322","30342012","K12 EY024225"],"pubmed_authors":["Boassa D","Mozafari M","Pormir CK","Toomey CB","Jung J","Painter C","Pflugmacher S","Park K","Rodriguez J","Weiser Novak S","Omran SP","Esko JD","Handa JT","Aastrup T","Gordts PLSM","Hauer J","Huang AS","Walker E","Pihl J","Jalali M"],"additional_accession":[]},"is_claimable":false,"name":"Bruch's membrane heparan sulfate retains lipoproteins in the early stages of age-related macular degeneration.","description":"Lipoprotein retention in Bruch's membrane is a key event in the pathobiology of early and intermediate age-related macular degeneration (AMD). However, the mechanism of lipoprotein retention in BrM is unknown. Given the established role of glycosaminoglycans (GAG) in binding lipoproteins, our laboratory sought to determine the role of GAGs in AMD BrM. In this study, BrM GAG content in AMD pathobiology was analyzed in human postmortem tissue. Strikingly, increased levels of highly sulfated heparan sulfate were present in AMD Bruch's membrane as compared to non-AMD samples. In addition, using scanning electron microscopy of postmortem AMD tissue, we show aggregates of lipoprotein-like particles on the retinal pigmented epithelium side of Bruch's membrane adjacent to heparan sulfate. We also ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-06-03T07:03:59.707Z","creation":"2026-04-25T03:22:01.859Z"},"accession":"S-EPMC12184332","cross_references":{"pubmed":["40512794"],"doi":["10.1073/pnas.2500727122"]}}