{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Khan GA"],"funding":["Deanship of Scientific Research, King Faisal University"],"pagination":["e0326312"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12185010"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(6)"],"pubmed_abstract":["Prostaglandin E2 (PGE2), that plays a crucial role in angiogenesis as well as in ischemic and inflammatory disorders of the brain, is associated with breakdown of the blood-brain barrier (BBB). Previously, we had shown that PGE2-induced human brain endothelial cells (HBECs) migration, and works in a cooperative manner through its three receptors (EP2, EP3 & EP4). However, the detailed signaling mechanism of PGE2-induced HBECs migration remains obscure. In this present study, we investigated the signaling pathway of actin dynamics/polymerization and migration of HBECs by PGE2 in vitro. Expression of ROCK was analyzed by ELISA and RT-PCR. Actin polymerization was evaluated by NBD-phallacidin immunofluorescence staining. HBECs expressed only ROCK II. PGE2 (100 pM) induced ROCK II expression o"],"journal":["PloS one"],"pubmed_title":["Prostaglandin E2 dependent migration of human brain endothelial cells is mediated through Rho-Kinase-II."],"pmcid":["PMC12185010"],"funding_grant_id":["KFU 252130 to GAK"],"pubmed_authors":["Ghosh A","Khan GA"],"additional_accession":[]},"is_claimable":false,"name":"Prostaglandin E2 dependent migration of human brain endothelial cells is mediated through Rho-Kinase-II.","description":"Prostaglandin E2 (PGE2), that plays a crucial role in angiogenesis as well as in ischemic and inflammatory disorders of the brain, is associated with breakdown of the blood-brain barrier (BBB). Previously, we had shown that PGE2-induced human brain endothelial cells (HBECs) migration, and works in a cooperative manner through its three receptors (EP2, EP3 & EP4). However, the detailed signaling mechanism of PGE2-induced HBECs migration remains obscure. In this present study, we investigated the signaling pathway of actin dynamics/polymerization and migration of HBECs by PGE2 in vitro. Expression of ROCK was analyzed by ELISA and RT-PCR. Actin polymerization was evaluated by NBD-phallacidin immunofluorescence staining. HBECs expressed only ROCK II. PGE2 (100 pM) induced ROCK II expression o","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-03T07:03:37.029Z","creation":"2026-04-25T03:21:58.034Z"},"accession":"S-EPMC12185010","cross_references":{"pubmed":["40549735"],"doi":["10.1371/journal.pone.0326312"]}}