biostudies-literatureKhan GADeanship of Scientific Research, King Faisal Universitye0326312https://www.ebi.ac.uk/biostudies/studies/S-EPMC12185010biostudies-literatureUnknown20(6)Prostaglandin E2 (PGE2), that plays a crucial role in angiogenesis as well as in ischemic and inflammatory disorders of the brain, is associated with breakdown of the blood-brain barrier (BBB). Previously, we had shown that PGE2-induced human brain endothelial cells (HBECs) migration, and works in a cooperative manner through its three receptors (EP2, EP3 & EP4). However, the detailed signaling mechanism of PGE2-induced HBECs migration remains obscure. In this present study, we investigated the signaling pathway of actin dynamics/polymerization and migration of HBECs by PGE2 in vitro. Expression of ROCK was analyzed by ELISA and RT-PCR. Actin polymerization was evaluated by NBD-phallacidin immunofluorescence staining. HBECs expressed only ROCK II. PGE2 (100 pM) induced ROCK II expression oPloS oneProstaglandin E2 dependent migration of human brain endothelial cells is mediated through Rho-Kinase-II.PMC12185010KFU 252130 to GAKGhosh AKhan GAfalseProstaglandin E2 dependent migration of human brain endothelial cells is mediated through Rho-Kinase-II.Prostaglandin E2 (PGE2), that plays a crucial role in angiogenesis as well as in ischemic and inflammatory disorders of the brain, is associated with breakdown of the blood-brain barrier (BBB). Previously, we had shown that PGE2-induced human brain endothelial cells (HBECs) migration, and works in a cooperative manner through its three receptors (EP2, EP3 & EP4). However, the detailed signaling mechanism of PGE2-induced HBECs migration remains obscure. In this present study, we investigated the signaling pathway of actin dynamics/polymerization and migration of HBECs by PGE2 in vitro. Expression of ROCK was analyzed by ELISA and RT-PCR. Actin polymerization was evaluated by NBD-phallacidin immunofluorescence staining. HBECs expressed only ROCK II. PGE2 (100 pM) induced ROCK II expression o2025-01-01T00:00:00Z20252026-06-03T07:03:37.029Z2026-04-25T03:21:58.034ZS-EPMC121850104054973510.1371/journal.pone.0326312