biostudies-literatureYu ZScience and Technology Innovation Project of the China Academy of Chinese Medical SciencesNational Key R&D Program of China802https://www.ebi.ac.uk/biostudies/studies/S-EPMC12195801biostudies-literatureUnknown18(6)<b>Background</b>: Yiqi Zengmian (YQZM) functions as an immunopotentiator by enhancing both cellular and humoral immunity. However, its pharmacodynamic active constituents, particularly those absorbed into the bloodstream, and mechanism of action remain unclear. This study aimed to investigate the immunopotentiating effects and mechanisms of YQZM in mice immunized with the BBIBP-CorV (Beijing Bio-Institute of Biological Products Coronavirus Vaccine). <b>Methods</b>: Serum pharmacochemistry and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were employed to identify bioavailable components of YQZM. The mice received the BBIBP-CorV twice on days 1 and 14, while YQZM was orally administered for 28 days. Neutralization assays and ELISA quantified antigen-specificPharmaceuticals (Basel, Switzerland)Mechanistic Exploration of Yiqi Zengmian in Regulating the Microenvironment as an Immunopotentiator with the Beijing Bio-Institute of Biological Products Coronavirus Vaccine Based on Transcriptomics and Integrated Serum Pharmacochemistry.PMC12195801CI2021A046052022YFC0867600Li HZheng XLi JWang AHao LHuo HHuang HHuang LLi WXiong YQiao YLi ZShan ZHuo RYu ZWang YLin LSun JDeng YLiu GfalseMechanistic Exploration of Yiqi Zengmian in Regulating the Microenvironment as an Immunopotentiator with the Beijing Bio-Institute of Biological Products Coronavirus Vaccine Based on Transcriptomics and Integrated Serum Pharmacochemistry.<b>Background</b>: Yiqi Zengmian (YQZM) functions as an immunopotentiator by enhancing both cellular and humoral immunity. However, its pharmacodynamic active constituents, particularly those absorbed into the bloodstream, and mechanism of action remain unclear. This study aimed to investigate the immunopotentiating effects and mechanisms of YQZM in mice immunized with the BBIBP-CorV (Beijing Bio-Institute of Biological Products Coronavirus Vaccine). <b>Methods</b>: Serum pharmacochemistry and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were employed to identify bioavailable components of YQZM. The mice received the BBIBP-CorV twice on days 1 and 14, while YQZM was orally administered for 28 days. Neutralization assays and ELISA quantified antigen-specific2025-01-01T00:00:00Z2025 May2026-05-26T20:10:44.877Z2026-05-26T03:07:13.66ZS-EPMC121958014057319810.3390/ph18060802