biostudies-literatureZhang LNational Natural Science Foundation of China for Young ScientistsSpecial Fund of Guangxi University of Chinese MedicineInnovation Project of Guangxi Graduate Education of GXUCM824https://www.ebi.ac.uk/biostudies/studies/S-EPMC12196505biostudies-literatureUnknown18(6)<b>Background/Objectives:</b><i>Camellia nitidissima</i> Chi (<i>C. nitidissima</i>), a traditional Chinese "food and medicine homology" plant, has demonstrated potential anti-tumor properties. However, its mechanisms of anti-lung cancer activity via ferroptosis remain unclear. This study aimed to construct an integrated research system of "natural product extraction-purification, bioactivity evaluation, and computational drug screening" to explore the bioactive compounds in <i>C. nitidissima</i> leaves targeting HMOX-1-mediated ferroptosis and their anti-lung cancer mechanisms. <b>Methods:</b> Active fractions were prepared using ethanol extraction combined with polyamide column chromatography. The anti-lung cancer activity was evaluated using the NCI-H1975 cell model. Ferroptosis was verPharmaceuticals (Basel, Switzerland)Identification of HMOX-1-Targeting Natural Compounds in &lt;i&gt;Camellia nitidissima&lt;/i&gt; Chi for NSCLC Therapy: Integrating Bioassay and In Silico Screening Approaches.PMC1219650582204756YCSY20230562025GXNSFAA069904Liang CZhang FLiang HXie JQin XDeng JZhang LDu ZHou XZhong MMo YHao EfalseIdentification of HMOX-1-Targeting Natural Compounds in &lt;i&gt;Camellia nitidissima&lt;/i&gt; Chi for NSCLC Therapy: Integrating Bioassay and In Silico Screening Approaches.<b>Background/Objectives:</b><i>Camellia nitidissima</i> Chi (<i>C. nitidissima</i>), a traditional Chinese "food and medicine homology" plant, has demonstrated potential anti-tumor properties. However, its mechanisms of anti-lung cancer activity via ferroptosis remain unclear. This study aimed to construct an integrated research system of "natural product extraction-purification, bioactivity evaluation, and computational drug screening" to explore the bioactive compounds in <i>C. nitidissima</i> leaves targeting HMOX-1-mediated ferroptosis and their anti-lung cancer mechanisms. <b>Methods:</b> Active fractions were prepared using ethanol extraction combined with polyamide column chromatography. The anti-lung cancer activity was evaluated using the NCI-H1975 cell model. Ferroptosis was ver2025-01-01T00:00:00Z2025 May2026-04-15T03:20:43.784Z2026-04-15T03:09:54.923ZS-EPMC121965054057321910.3390/ph18060824