biostudies-literatureUnknown75(6)Zheng YYSix Gram-staining-negative, aerobic, non-motile and rod-shaped bacterial strains, designated as D3-2-R+10T, C2-6-M1T, E1-3-M2, D2-2-M3, D2-2-M2 and D2-2-M1, were isolated from the shore soil of LungmuCo Lake in Xizang Autonomous Region, China. The 16S rRNA gene sequence comparisons confirmed that the six isolates belonged to the genus Algoriphagus of the family Cyclobacteriaceae. The 16S rRNA gene sequence of strains D3-2-R+10T, D2-2-M3, D2-2-M2 and D2-2-M1 exhibited the highest similarities to the type strains of Algoriphagus antarcticus LMG 21980T(98.1-98.5 %) and Algoriphagus resistens NH1T (97.9-98.4 %). Meanwhile, the 16S rRNA gene sequence of strains C2-6-M1T and E1-3-M2 showed the highest similarities to A. antarcticus LMG 21980T (98.2-98.7%). The average nt identity and digital DNA-DNA hybridization values among strains D3-2-R+10T and C2-6-M1T and their most closely related species A. antarcticus LMG 21980T were all lower than the threshold values for delineating species, indicating that they represent two separate novel species of Algoriphagus. The predominant cellular fatty acids of strains D3-2-R+10T and C2-6-M1T included iso-C15 : 0, summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c) and summed feature 9 (iso-C17 : 1 ω9c/C16 : 0 10-methyl), and the predominant respiratory quinone was MK-7. The major polar lipids of strain D3-2-R+10T comprised phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids, while those of strain C2-6-M1T included phosphatidylethanolamine, two unidentified phospholipids and seven unidentified lipids. The genome size of strains D3-2-R+10T and C2-6-M1T was 6.0 and 5.0 Mb, respectively, with the DNA G+C contents of 40.1 and 40.9 mol %. Based on the above descriptions, the six strains were identified as two novel species of the genus Algoriphagus, for which the names Algoriphagus aurantiacus sp. nov. and Algoriphagus persicinus sp. nov. were proposed, with the type strains, respectively.International journal of systematic and evolutionary microbiologyhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC12205151biostudies-literatureAlgoriphagus aurantiacus sp. nov. and Algoriphagus persicinus sp. nov., two novel species isolated from the shore soil of salt lake.PMC12205151Wang RLi AHZhang XZheng YYLiu ZXPhurbu DfalseAlgoriphagus aurantiacus sp. nov. and Algoriphagus persicinus sp. nov., two novel species isolated from the shore soil of salt lake.Six Gram-staining-negative, aerobic, non-motile and rod-shaped bacterial strains, designated as D3-2-R+10T, C2-6-M1T, E1-3-M2, D2-2-M3, D2-2-M2 and D2-2-M1, were isolated from the shore soil of LungmuCo Lake in Xizang Autonomous Region, China. The 16S rRNA gene sequence comparisons confirmed that the six isolates belonged to the genus Algoriphagus of the family Cyclobacteriaceae. The 16S rRNA gene sequence of strains D3-2-R+10T, D2-2-M3, D2-2-M2 and D2-2-M1 exhibited the highest similarities to the type strains of Algoriphagus antarcticus LMG 21980T(98.1-98.5 %) and Algoriphagus resistens NH1T (97.9-98.4 %). Meanwhile, the 16S rRNA gene sequence of strains C2-6-M1T and E1-3-M2 showed the highest similarities to A. antarcticus LMG 21980T (98.2-98.7%). The average nt identity and digital DNA-DNA hybridization values among strains D3-2-R+10T and C2-6-M1T and their most closely related species A. antarcticus LMG 21980T were all lower than the threshold values for delineating species, indicating that they represent two separate novel species of Algoriphagus. The predominant cellular fatty acids of strains D3-2-R+10T and C2-6-M1T included iso-C15 : 0, summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c) and summed feature 9 (iso-C17 : 1 ω9c/C16 : 0 10-methyl), and the predominant respiratory quinone was MK-7. The major polar lipids of strain D3-2-R+10T comprised phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids, while those of strain C2-6-M1T included phosphatidylethanolamine, two unidentified phospholipids and seven unidentified lipids. The genome size of strains D3-2-R+10T and C2-6-M1T was 6.0 and 5.0 Mb, respectively, with the DNA G+C contents of 40.1 and 40.9 mol %. Based on the above descriptions, the six strains were identified as two novel species of the genus Algoriphagus, for which the names Algoriphagus aurantiacus sp. nov. and Algoriphagus persicinus sp. nov. were proposed, with the type strains, respectively.2025-01-01T00:00:00Z2025 Jun2026-06-01T15:55:43.089Z2026-04-08T13:51:58.647ZS-EPMC122051514057704210.1099/ijsem.0.006826