biostudies-literatureCosta SNNIA NIH HHSNIDDK NIH HHSNIGMS NIH HHS1591280https://www.ebi.ac.uk/biostudies/studies/S-EPMC12206641biostudies-literatureUnknown16Expansion of bone marrow (BM) adipocytes has been linked to nutritional pressures, suggesting that BM is a dynamic compartment that responds to fluctuations in systemic nutritional availability to regulate osteogenesis and hematopoiesis. Here, we investigated BM metabolism in response to acute overnutrition (high calorie diet; HCD) and calorie deprivation (fasting). Participants underwent a 10-day HCD followed by a two-week interval of an ad libitum diet and then underwent 10 days of fasting. BM adipocytes and sera were collected before and after each dietary intervention for each participant. Using comprehensive and integrated analyses, we characterized nutritional influences on BM adiposity. BM adipocytes after HCD showed an upregulation of <i>FOXP3</i>, the transcription factor that conFrontiers in endocrinologyAltered metabolomics and inflammatory transcriptomics in human bone marrow adipocytes after acute high calorie diet and acute fasting.PMC12206641P20 GM103449P20 GM121301U19 AG060917P30 DK040561R24 DK084970U54 GM115516Pachon-Pena GFazeli PKDragon JTighe SBredella MAVary CCosta SNRosen CJfalseAltered metabolomics and inflammatory transcriptomics in human bone marrow adipocytes after acute high calorie diet and acute fasting.Expansion of bone marrow (BM) adipocytes has been linked to nutritional pressures, suggesting that BM is a dynamic compartment that responds to fluctuations in systemic nutritional availability to regulate osteogenesis and hematopoiesis. Here, we investigated BM metabolism in response to acute overnutrition (high calorie diet; HCD) and calorie deprivation (fasting). Participants underwent a 10-day HCD followed by a two-week interval of an ad libitum diet and then underwent 10 days of fasting. BM adipocytes and sera were collected before and after each dietary intervention for each participant. Using comprehensive and integrated analyses, we characterized nutritional influences on BM adiposity. BM adipocytes after HCD showed an upregulation of <i>FOXP3</i>, the transcription factor that con2025-01-01T00:00:00Z20252026-06-06T15:14:02.524Z2026-06-01T03:11:07.207ZS-EPMC122066414058951810.3389/fendo.2025.1591280