biostudies-literatureUnknown13(7)Preuss LGlobal plastic pollution in oceans and estuaries is increasing rapidly, and it is well known that bacteria colonize plastic particles of all sizes. Vibrio spp. are frequently found as part of the plastisphere. We recently showed that Vibrio gazogenes DSM 21264 harbors a promiscuous esterase designated PET6. We now provide evidence that the pet6 gene is expressed under a wide range of environmental conditions in its native host. However, in PET- and PE-grown biofilms, the pet6 gene expression was not affected by the type of surface. The pet6 transcription was sufficient to allow enzyme production and release of µM amounts of mono-(2-hydroxyethyl) terephthalate (MHET) and terephthalic acid (TPA) already after 24 hours of incubation on the PET foil. Notably,Microbiology spectrume0018125https://www.ebi.ac.uk/biostudies/studies/S-EPMC12211005biostudies-literaturePolyethylene terephthalate (PET) primary degradation products affect c-di-GMP-, cAMP-signaling, and quorum sensing (QS) in Vibrio gazogenes DSM 21264.PMC12211005Burmeister NDumnitch AMaison WDaniel RVollstedt CStreit WRPreuss LAlawi MPoehlein ATrinh LfalsePolyethylene terephthalate (PET) primary degradation products affect c-di-GMP-, cAMP-signaling, and quorum sensing (QS) in Vibrio gazogenes DSM 21264.Global plastic pollution in oceans and estuaries is increasing rapidly, and it is well known that bacteria colonize plastic particles of all sizes. Vibrio spp. are frequently found as part of the plastisphere. We recently showed that Vibrio gazogenes DSM 21264 harbors a promiscuous esterase designated PET6. We now provide evidence that the pet6 gene is expressed under a wide range of environmental conditions in its native host. However, in PET- and PE-grown biofilms, the pet6 gene expression was not affected by the type of surface. The pet6 transcription was sufficient to allow enzyme production and release of µM amounts of mono-(2-hydroxyethyl) terephthalate (MHET) and terephthalic acid (TPA) already after 24 hours of incubation on the PET foil. Notably,2025-01-01T00:00:00Z2025 Jul2026-06-02T17:49:00.93Z2026-05-27T03:07:55.25ZS-EPMC122110054048846810.1128/spectrum.00181-25