{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(7)"],"submitter":["Deng Z"],"pubmed_abstract":["Tuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 <i>Trans</i> Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen detection, was constrained by its reliance on specialized fluorescence equipment and lack of visual output, limiting its use in resource-limited settings. To overcome these limitations, we introduce an innovative RPA/CRISPR-Cas12f1_ge4.1 dual-mode system for rapid <i>Mycobacterium tuberculosis</i> detection. This system combines engineered Cas12f_ge4.1 with recombinase polymerase amplification (RPA), offering both fluorescent and lateral flow detection. It achieves high sensitivity with detection li"],"journal":["Microbiology spectrum"],"pagination":["e0265224"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12211024"],"repository":["biostudies-literature"],"pubmed_title":["Rapid and sensitive detection of &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt; using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts."],"pmcid":["PMC12211024"],"pubmed_authors":["Liu H","He J","Weng X","Deng Z","Luo G","Wen P","Zou T","Zhou X","Gan T","Tang H"],"additional_accession":[]},"is_claimable":false,"name":"Rapid and sensitive detection of &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt; using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts.","description":"Tuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 <i>Trans</i> Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen detection, was constrained by its reliance on specialized fluorescence equipment and lack of visual output, limiting its use in resource-limited settings. To overcome these limitations, we introduce an innovative RPA/CRISPR-Cas12f1_ge4.1 dual-mode system for rapid <i>Mycobacterium tuberculosis</i> detection. This system combines engineered Cas12f_ge4.1 with recombinase polymerase amplification (RPA), offering both fluorescent and lateral flow detection. It achieves high sensitivity with detection li","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-06-01T15:40:35.563Z","creation":"2026-04-08T13:44:10.867Z"},"accession":"S-EPMC12211024","cross_references":{"pubmed":["40488470"],"doi":["10.1128/spectrum.02652-24"]}}