biostudies-literatureUnknown13(7)Deng ZTuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 <i>Trans</i> Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen detection, was constrained by its reliance on specialized fluorescence equipment and lack of visual output, limiting its use in resource-limited settings. To overcome these limitations, we introduce an innovative RPA/CRISPR-Cas12f1_ge4.1 dual-mode system for rapid <i>Mycobacterium tuberculosis</i> detection. This system combines engineered Cas12f_ge4.1 with recombinase polymerase amplification (RPA), offering both fluorescent and lateral flow detection. It achieves high sensitivity with detection liMicrobiology spectrume0265224https://www.ebi.ac.uk/biostudies/studies/S-EPMC12211024biostudies-literatureRapid and sensitive detection of <i>Mycobacterium tuberculosis</i> using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts.PMC12211024Liu HHe JWeng XDeng ZLuo GWen PZou TZhou XGan TTang HfalseRapid and sensitive detection of <i>Mycobacterium tuberculosis</i> using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts.Tuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 <i>Trans</i> Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen detection, was constrained by its reliance on specialized fluorescence equipment and lack of visual output, limiting its use in resource-limited settings. To overcome these limitations, we introduce an innovative RPA/CRISPR-Cas12f1_ge4.1 dual-mode system for rapid <i>Mycobacterium tuberculosis</i> detection. This system combines engineered Cas12f_ge4.1 with recombinase polymerase amplification (RPA), offering both fluorescent and lateral flow detection. It achieves high sensitivity with detection li2025-01-01T00:00:00Z2025 Jul2026-06-01T15:40:35.563Z2026-04-08T13:44:10.867ZS-EPMC122110244048847010.1128/spectrum.02652-24